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Ascentis® Express C8, 2 μm UHPLC Column

2 μm particle size, L × I.D. 2 cm × 2.1 mm



stainless steel column

Nivel de calidad



suitable for USP L7

línea de producto







1 ea of


1000 bar max. pressure (14500 psi)
60 °C temp. range


LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × D.I.

2 cm × 2.1 mm


<5 ppm (metals)


Fused-Core particle platform
superficially porous particle

grupo activo de la matriz

dimethyloctyl phase

tamaño de partícula

2 μm

tamaño de poro

90 Å pore size


120 m2/g




food and beverages

técnica de separación

reversed phase

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Descripción general

Ascentis® Express 2μm C8 UHPLC columns are based on a 90 Å Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4-micron thick porous shell and the small overall particle size of 2-microns. The densely bonded, extensively endcapped dimethyloctyl stationary phase of Ascentis Express 2μm C8 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

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Información legal

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
Ugandar Reddy Inugala et al.
Journal of chromatographic science, 51(5), 453-459 (2012-10-13)
This paper describes the development of a rapid, novel, stability-indicating gradient reversed-phase high-performance liquid chromatographic method and associated system suitability parameters for the analysis of naproxcinod in the presence of its related substances and degradents using a quality-by-design approach. All
Luigi Silvestro et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(30), 3134-3142 (2010-10-20)
Quantitative methods using LC-MS/MS allow achievement of adequate sensitivity for pharmacokinetic studies with clopidogrel; three such methods, with LLOQs as low as 5 pg/mL, were developed and fully validated according to the well established FDA 2001 guidelines. The chromatographic separations
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 81-82, 126-132 (2013-05-07)
A closed-vessel microwave-assisted extraction (MAE) technique was optimized for the first time for the extraction of polyphenols from raw propolis. The results obtained by means of response surface experimental design methodology showed that the best global response was reached when
Han Young Eom et al.
Journal of chromatography. A, 1217(26), 4347-4354 (2010-05-11)
Saikosaponins are triterpene saponins derived from the roots of Bupleurum falcatum L. (Umbelliferae), which has been traditionally used to treat fever, inflammation, liver diseases, and nephritis. It is difficult to analyze saikosaponins using HPLC-UV due to the lack of chromophores.


Ascentis® Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

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