Merck
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568223-U

Supelco

Discovery® BIO Wide Pore C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

eCl@ss:
32110501
NACRES:
SB.52

material

stainless steel column

Nivel de calidad

100

Agency

suitable for USP L1

Línea del producto

Discovery®

característica

endcapped

manufacturer/tradename

Discovery®

envase

1 ea of

extensión del etiquetado

9.2% Carbon loading

parámetro

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × D.I.

25 cm × 4.6 mm

cobertura de la superficie

3.6 μmol/m2

matriz

silica particle platform
fully porous particle

grupo activo de la matriz

C18 (octadecyl) phase

tamaño de partícula

5 μm

tamaño de poro

300 Å

superficie

100 m2/g

operating pH

2-8

técnica de separación

reversed phase

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Descripción general

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

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Información legal

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

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Certificate of Origin

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