FLAG® tags enable superior detection and robust purification of recombinant fusion proteins, with proven utility in numerous downstream applications from binding and activity assays to structural analysis. The FLAG® epitope tag is a short, hydrophilic, eight-amino acid peptide (DYKDDDDK tag) that is readily cleavable by enterokinase (EK). Due to its small size and hydrophilic nature, the FLAG® tag commonly resides on the surface of the fusion protein, minimizing effects on function, secretion, or transport of the fusion protein. Regardless of your final application, we have the tools needed to express, purify, and detect FLAG® fusion proteins.
Our FLAG® tag protein expression portfolio includes vectors for efficient expression of recombinant fusion proteins in bacterial and mammalian systems. SnapFast™ vector technology allows you to easily move the gene-of-interest from one vector to another for more efficient and cost-effective cloning.
We offer a variety of agarose affinity purification gels, magnetic beads, purification kits, and coated plates for isolation and purification of FLAG® tagged proteins.
FLAG® and 3xFLAG™ tags include binding sites for highly specific anti-FLAG® monoclonal antibodies as well as polyclonal antibodies and conjugates for ELISA, blotting applications, immunofluorescence, immunocytochemistry, immunohistochemistry (IHC), blotting, and flow cytometry.
*IP=immunoprecipitation; IC=immunochemistry, WB=western blot, EIA=enzyme-linked immunosorbent assay (ELISA)