Showing 1-30 of 32 resultados for "


Mengfan Tang et al.
The Journal of biological chemistry, 289(49), 34024-34032 (2014-10-09)
Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) has been shown to be involved in gene silencing and DNA damage. However, the exact mechanisms of how SMCHD1 participates in DNA damage remains largely unknown. Here we present evidence...
Xin-Jiang Zhu et al.
Molecular medicine reports, 9(6), 2283-2292 (2014-04-11)
The present study aimed to investigate the role of histone modification and DNA methylation in epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) silencing in gastric cancer (GC). In the present study, four GC cell lines, and 45 paired...
Xu Wang et al.
The EMBO journal, 36(9), 1243-1260 (2017-03-23)
Enhancer of zeste homolog 2 (EZH2) has been characterized as a critical oncogene and a promising drug target in human malignant tumors. The current EZH2 inhibitors strongly suppress the enhanced enzymatic function of mutant EZH2 in some lymphomas. However, the...
Adam E Handel et al.
Frontiers in immunology, 9, 2120-2120 (2018-10-05)
Thymic epithelial cells (TEC) effect crucial roles in thymopoiesis including the control of negative thymocyte selection. This process depends on their capacity to express promiscuously genes encoding tissue-restricted antigens. This competence is accomplished in medullary TEC (mTEC) in part by...
Manjeet K Rao et al.
The Journal of biological chemistry, 289(51), 35087-35101 (2014-10-22)
Genome-wide studies have revealed that genes commonly have a high density of RNA polymerase II just downstream of the transcription start site. This has raised the possibility that genes are commonly regulated by transcriptional elongation, but this remains largely untested...
Hui Li et al.
PLoS genetics, 7(6), e1002122-e1002122 (2011-06-23)
Eukaryotic nuclei contain regions of differentially staining chromatin (heterochromatin), which remain condensed throughout the cell cycle and are largely transcriptionally silent. RNAi knockdown of the highly conserved heterochromatin protein HP1 in Drosophila was previously shown to preferentially reduce male viability....
Xinjiang Zhu et al.
International journal of oncology, 46(1), 195-204 (2014-10-31)
The Pleckstrin and Sec7 domain-containing (PSD) gene has been recently found to participate in the progression of several types of cancer. In the present study, we identified PSD as a candidate tumor suppressor gene silenced through epigenetic modification in gastric...
Yu Zhang et al.
The Journal of biological chemistry, 286(49), 42414-42425 (2011-10-20)
Polycomb group proteins play essential roles in transcriptional regulation of multiple gene families involved in various pathophysiological processes. It is believed that Polycomb Repressive Complex 2 (PRC2) is targeted to chromatin by the EED subunit to methylate histone H3 lysine...
Xi-Yong Yu et al.
Molecular biology reports, 39(9), 8891-8898 (2012-06-19)
Diabetic patients continue to develop inflammation and cardiovascular complication even after achieving glycemic control, suggesting a "metabolic memory". Metabolic memory is a major challenge in the treatment of diabetic complication, and the mechanisms underlying metabolic memory are not clear. Recent...
Emilie Lukášová et al.
FEBS open bio, 9(5), 870-890 (2019-04-15)
Replication stress (RS) is a major driver of genomic instability and tumorigenesis. Here, we investigated whether RS induced by the nucleotide analog fludarabine and specific kinase inhibitors [e.g. targeting checkpoint kinase 1 (Chk1) or ataxia telangiectasia and Rad3-related (ATR)] led...
Fei Teng et al.
Oncotarget, 7(33), 53571-53582 (2016-07-23)
A central aim in cancer research is to identify genes with altered expression patterns in tumor specimens and their potential role in tumorigenesis. Most types of tumors, including hepatocellular carcinoma (HCC), are heterogeneous in terms of genotype and phenotype. Thus...
J K Wiencke et al.
Oncogene, 27(17), 2412-2421 (2007-10-31)
Histone H3 lysine 9 trimethylation (H3K9Me3) has been associated with transcriptional repression, but recent findings implicate this chromatin modification in transcriptional activation and mRNA elongation by RNA polymerase II. Here, we applied immunoprecipitation (IP) with a custom DNA tiling microarray...
Julio Aguado et al.
Nature communications, 10(1), 4990-4990 (2019-11-20)
Hutchinson-Gilford progeria syndrome (HGPS) is a genetic disorder characterized by premature aging features. Cells from HGPS patients express progerin, a truncated form of Lamin A, which perturbs cellular homeostasis leading to nuclear shape alterations, genome instability, heterochromatin loss, telomere dysfunction...
Jacob D Jaffe et al.
Nature genetics, 45(11), 1386-1391 (2013-10-01)
Epigenetic dysregulation is an emerging hallmark of cancers. We developed a high-information-content mass spectrometry approach to profile global histone modifications in human cancers. When applied to 115 lines from the Cancer Cell Line Encyclopedia, this approach identified distinct molecular chromatin...
Liver-specific knockout of histone methyltransferase G9a impairs liver maturation and dysregulates inflammatory, cytoprotective, and drug-processing genes.
Lu, et al.
Xenobiotica, 49, 740-752 (2020)
Constantinos Chronis et al.
Cell, 168(3), 442-459 (2017-01-24)
Oct4, Sox2, Klf4, and cMyc (OSKM) reprogram somatic cells to pluripotency. To gain a mechanistic understanding of their function, we mapped OSKM-binding, stage-specific transcription factors (TFs), and chromatin states in discrete reprogramming stages and performed loss- and gain-of-function experiments. We...
Hung-Fu Liao et al.
Reproduction (Cambridge, England), 150(4), 245-256 (2015-07-15)
Nuclear transfer (NT) is a technique used to investigate the development and reprogramming potential of a single cell. DNA methyltransferase-3-like, which has been characterized as a repressive transcriptional regulator, is expressed in naturally fertilized egg and morula/blastocyst at pre-implantation stages....
Emmanuelle Deniaud et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 25(3-4), 227-239 (2017-05-10)
In the premature ageing disease Hutchinson-Gilford progeria syndrome (HGPS), the underlying genetic defect in the lamin A gene leads to accumulation at the nuclear lamina of progerin-a mutant form of lamin A that cannot be correctly processed. This has been...
Kirk J McManus et al.
The Journal of biological chemistry, 281(13), 8888-8897 (2005-12-24)
Histone methylation is unique among post-translational histone modifications by virtue of its stability. It is thought to be a relatively stable and heritable epigenetic mark for gene-specific regulation. In this study, we use quantitative in situ approaches to investigate the...
Shicheng Su et al.
Nature communications, 6, 8523-8523 (2015-10-06)
Macrophages play a pivotal role in tissue fibrogenesis, which underlies the pathogenesis of many end-stage chronic inflammatory diseases. MicroRNAs are key regulators of immune cell functions, but their roles in macrophage's fibrogenesis have not been characterized. Here we show that...
Qinghao Zhang et al.
PloS one, 9(1), e84925-e84925 (2014-01-16)
Hepatocyte nuclear factor 4α (HNF4α) is a liver-enriched transcription factor essential for liver development and function. In hepatocytes, HNF4α regulates a large number of genes important for nutrient/xenobiotic metabolism and cell differentiation and proliferation. Currently, little is known about the...
Suv39h1 mediates AP-2?-dependent inhibition of C/EBP? expression during adipogenesis.
Zhang, ZC; Liu, Y; Li, SF; Guo, L; Zhao, Y; Qian, SW; Wen, B; Tang, QQ; Li, X
Molecular and cellular biology null
Jin Hou et al.
Cancer cell, 19(2), 232-243 (2011-02-15)
The full scale of human miRNome in specific cell or tissue, especially in cancers, remains to be determined. An in-depth analysis of miRNomes in human normal liver, hepatitis liver, and hepatocellular carcinoma (HCC) was carried out in this study. We...
Nicholas J Brideau et al.
Molecular and cellular biology, 35(23), 4053-4068 (2015-09-24)
The chromosomal protein SMCHD1 plays an important role in epigenetic silencing at diverse loci, including the inactive X chromosome, imprinted genes, and the facioscapulohumeral muscular dystrophy locus. Although homology with canonical SMC family proteins suggests a role in chromosome organization...
Sathish Kumar Mungamuri et al.
Nature structural & molecular biology, 19(5), 478-484 (2012-04-03)
p53 is a major sensor of cellular stresses, and its activation influences cell fate decisions. We identified SUV39H1, a histone code 'writer' responsible for the histone H3 Lys9 trimethylation (H3K9me3) mark for 'closed' chromatin conformation, as a target of p53...
Pengpeng Ma et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(8), E481-E489 (2012-01-10)
Dramatic changes in chromatin structure and histone modification occur during oocyte growth, as well as a global cessation of transcription. The role of histone modifications in these processes is poorly understood. We report the effect of conditionally deleting Hdac1 and...
Mario R Calderon et al.
Nucleic acids research, 42(11), 7012-7027 (2014-05-16)
We identified a novel interaction between ligand-dependent corepressor (LCoR) and the corepressor KRAB-associated protein-1 (KAP-1). The two form a complex with C2H2 zinc-finger transcription factor ZBRK1 on an intronic binding site in the growth arrest and DNA-damage-inducible α (GADD45A) gene...
Cai Qi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(13), 4494-4508 (2014-03-29)
Dendritic arborization is one of the key determinants of precise circuits for information processing in neurons. Unraveling the molecular mechanisms underlying dendrite morphogenesis is critical to understanding the establishment of neuronal connections. Here, using gain- and loss-of-function approaches, we defined...
Melissa Suter et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(2), 714-726 (2010-11-26)
The effect of in utero exposure to a maternal high-fat diet on the peripheral circadian system of the fetus is unknown. Using mRNA copy number analysis, we report that the components of the peripheral circadian machinery are transcribed in the...