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95437

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Mathieu Taveau et al.
Molecular and cellular biology, 23(24), 9127-9135 (2003-12-04)
Calpain 3 (Capn3) is known as the skeletal muscle-specific member of the calpains, a family of intracellular nonlysosomal cysteine proteases. This enigmatic protease has many unique features among the calpain family and, importantly, mutations in Capn3 have been shown to
Angela Fülbier et al.
Stem cell research & therapy, 5(5), 113-113 (2014-10-11)
Adipose-derived stroma cells (ASCs) are attractive cells for cell-based gene therapy but are generally difficult to transfect. Nucleofection has proven to be an efficient method for transfection of primary cells. Therefore, we used this technique to transfect ASCs with a
Tiago Sousa et al.
Journal of pharmaceutical sciences, 103(10), 3171-3175 (2014-08-06)
Azo-bonded prodrugs of 5-aminosalicylic acid (mesalazine)-sulfasalazine, balsalazide, and olsalazine, which are used in the treatment of ulcerative colitis, rely on colonic bacteria to cleave the azo bond and liberate the active drug in the large intestine. The aim of this
Alok Pandey et al.
The Journal of biological chemistry, 288(52), 36773-36786 (2013-11-13)
For iron-sulfur (Fe-S) cluster synthesis in mitochondria, the sulfur is derived from the amino acid cysteine by the cysteine desulfurase activity of Nfs1. The enzyme binds the substrate cysteine in the pyridoxal phosphate-containing site, and a persulfide is formed on
Yoshiaki Nishiya et al.
Bioscience, biotechnology, and biochemistry, 69(11), 2244-2246 (2005-11-25)
We have developed a new enzymatic assay for determining L-cysteine concentration. The method involves the use of betaC-S lyase from Streptococcus anginosus, which catalyzes the alpha,beta-elimination of L-cysteine to hydrogen sulfide, pyruvate, and ammonia. The production of pyruvate is measured
Masaru Wada et al.
Applied microbiology and biotechnology, 73(1), 48-54 (2006-10-06)
L-Cysteine is an important amino acid both biologically and commercially. Although most amino acids are commercially produced by fermentation, cysteine is mainly produced by protein hydrolysis. However, synthetic or biotechnological products have been preferred in the market. Biotechnological processes for
N M Kredich et al.
Ciba Foundation symposium, (72)(72), 87-99 (1979-01-01)
In Salmonella typhimurium and Escherichia coli the biosynthesis of L-cysteine from L-serine and inorganic sulphate proceeds along a branched convergent pathway along one arm of which sulphate is reduced to sulphide, while on the other L-serine is acetylated to O-acetyl-L-serine.
Jennifer Bridwell-Rabb et al.
Biochemistry, 53(30), 4904-4913 (2014-06-28)
Iron-sulfur clusters are ubiquitous protein cofactors with critical cellular functions. The mitochondrial Fe-S assembly complex, which consists of the cysteine desulfurase NFS1 and its accessory protein (ISD11), the Fe-S assembly protein (ISCU2), and frataxin (FXN), converts substrates l-cysteine, ferrous iron
Koryn A Carver et al.
American journal of physiology. Cell physiology, 307(11), C989-C998 (2014-07-25)
Mammals have circadian variation in blood pressure, heart rate, vascular tone, thrombotic tendency, and cerebral blood flow (CBF). These changes may be in part orchestrated by circadian variation in clock gene expression within cells comprising the vasculature that modulate blood
Clare Abbenhardt et al.
The Journal of nutrition, 144(5), 714-721 (2014-03-22)
Folate-mediated one-carbon metabolism is essential for DNA synthesis, repair, and methylation. Perturbations in one-carbon metabolism have been implicated in increased risk of some cancers and may also affect inflammatory processes. We investigated these interrelated pathways to understand their relation. The
Matthias Fellner et al.
Analytical biochemistry, 459, 56-60 (2014-05-27)
A fast and easy method for enzyme activity assays using the chromogenic Ellman reagent, 5,5'-dithiobis(2-nitrobenzoic acid), was developed. The method was used to measure the activity of the nonheme mono-iron enzyme cysteine dioxygenase. Quantifying the depletion of the substrate, cysteine
T M Jeitner et al.
Journal of neurochemistry, 79(5), 1109-1112 (2001-12-12)
Pathological-length polyglutamine (Q(n)) expansions, such as those that occur in the huntingtin protein (htt) in Huntington's disease (HD), are excellent substrates for tissue transglutaminase in vitro, and transglutaminase activity is increased in post-mortem HD brain. However, direct evidence for the
Carolina Pereira Tavares et al.
Biochimica et biophysica acta, 1844(4), 810-817 (2014-03-04)
MYB proteins are a family of transcription factors that play an important role in plant development and regulatory defense processes. Arabidopsis thaliana MYB30 (AtMYB30), a member of this protein family, is involved in cell death processes during the hypersensitive response
Byung Sun Park et al.
Journal of neurochemistry, 132(2), 230-242 (2014-08-16)
Hydrogen sulfide (H2 S) functions as a physiological gas transmitter in both normal and pathophysiological cellular events. H2 S is produced from substances by three enzymes: cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MST). In human tissues, these
Tomer Meir et al.
Kidney international, 86(6), 1106-1115 (2014-06-19)
Parathyroid hormone (PTH) increases FGF23 mRNA and protein levels in vivo and in vitro. Here we tested whether the increased FGF23 expression by PTH is mediated by the orphan nuclear receptor Nurr1. PTH increased Nurr1 mRNA levels prior to elevation
C Syburra et al.
Ukrains'kyi biokhimichnyi zhurnal (1999 ), 71(3), 112-115 (1999-12-28)
It is well known that brain and nervous system cells are prone to oxidative damage because of their relatively low content of antioxidants, especially enzymatic ones, and of the high levels of both membrane polyunsaturated fatty acids (PUFA) and iron
Joanna Czarnecka et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 822(1-2), 85-90 (2005-07-05)
New method of qualitative and quantitative analysis of nucleotides in human cerebrospinal fluid (CSF), based on the combination of extraction of purines and pyrimidines to the solid phase (SPE) and high-performance liquid chromatography (HPLC), was proposed. Use of SPE and
Tobias Piroth et al.
Cell transplantation, 23(8), 995-1007 (2013-05-03)
Restorative cell therapy concepts in neurodegenerative diseases are aimed at replacing lost neurons. Despite advances in research on pluripotent stem cells, fetal tissue from routine elective abortions is still regarded as the only safe cell source. Progenitor cells isolated from
Y Zhang et al.
British journal of cancer, 110(1), 71-82 (2013-12-11)
Heat shock protein 90 (HSP90) has a key role in the maintenance of the cellular proteostasis. However, HSP90 is also involved in stabilisation of oncogenic client proteins and facilitates oncogene addiction and cancer cell survival. The development of HSP90 inhibitors
David Balchin et al.
Biochemistry, 52(51), 9394-9402 (2013-11-26)
Protein S-nitrosation is a post-translational modification that regulates the function of more than 500 human proteins. Despite its apparent physiological significance, S-nitrosation is poorly understood at a molecular level. Here, we investigated the effect of S-nitrosation on the activity, structure
Huifang Xu et al.
The Journal of biological chemistry, 289(19), 13565-13574 (2014-03-29)
The discoidin domain receptors, DDR1 and DDR2, are receptor tyrosine kinases that are activated by collagen. DDR activation does not appear to occur by the common mechanism of ligand-induced receptor dimerization: the DDRs form stable noncovalent dimers in the absence
Maria V Yelshanskaya et al.
Science (New York, N.Y.), 345(6200), 1070-1074 (2014-08-12)
Ionotropic glutamate receptors (iGluRs) mediate most excitatory neurotransmission in the central nervous system and function by opening their ion channel in response to binding of agonist glutamate. Here, we report a structure of a homotetrameric rat GluA2 receptor in complex
Z Li et al.
Letters in applied microbiology, 59(6), 615-620 (2014-08-26)
Cysteine phytase is the main phytate-degrading enzyme of ruminant animals. To explore the genetic diversity and dynamic expression profile of cysteine phytase in sheep rumen during a feeding cycle, four transcript (0, 4, 9 and 16 h after feeding) and one
Hong Sik Yoo et al.
Journal of toxicology and environmental health. Part A, 78(1), 32-49 (2014-11-27)
Trichloroethylene (TCE) is a well-known environmental and occupational toxicant that is classified as carcinogenic to humans based on the epidemiological evidence of an association with higher risk of renal-cell carcinoma. A number of scientific issues critical for assessing human health
Alan Tseng et al.
Cartilage, 5(4), 241-251 (2015-06-13)
Our goal was to engineer cartilage in vivo using auricular chondrocytes that underwent clinically relevant expansion and using methodologies that could be easily translated into health care practice. Sheep and human chondrocytes were isolated from auricular cartilage biopsies and expanded
Luc A Cynober
Nutrition (Burbank, Los Angeles County, Calif.), 18(9), 761-766 (2002-09-26)
The plasma concentration of an amino acid (AA) is the result of its rates of appearance (Ra) in and disappearance (Rd) from plasma. As for most nutrients, AA Ra and Rd are tightly regulated and at the postabsorptive state Ra
Rebecca Lamb et al.
Oncotarget, 6(7), 4585-4601 (2015-02-12)
We have used an unbiased proteomic profiling strategy to identify new potential therapeutic targets in tumor-initiating cells (TICs), a.k.a., cancer stem cells (CSCs). Towards this end, the proteomes of mammospheres from two breast cancer cell lines were directly compared to
Nitric oxide quenches the fire in heart mitochondria.
Paul T Schumacker
Nature medicine, 19(6), 666-667 (2013-06-08)
Pravin Kesarwani et al.
Cancer research, 74(21), 6036-6047 (2014-08-29)
Ex vivo-expanded CD8(+) T cells used for adoptive immunotherapy generally acquire an effector memory-like phenotype (TEM cells). With regard to therapeutic applications, two undesired features of this phenotype in vivo are limited persistence and reduced antitumor efficacy, relative to CD8(+)
Jingjing Liu et al.
Chemical research in toxicology, 28(3), 394-400 (2014-10-18)
Carcinogenic formaldehyde is produced by endogenous protein oxidation and various exogenous sources. With formaldehyde being both ubiquitous in the ambient environment and one of the most common reactive carbonyls produced from endogenous metabolism, quantifying formaldehyde exposure is an essential step
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