Protein pulldown

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Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles

Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Agarose Beads Vs. Magnetic Beads in ChIP

Agarose beads Vs. Magnetic beads in Chromatin Immunoprecipitation (ChIP)

Centrifugation Separations

CsCl gradient centrifugation separates RNA from DNA; differential and density gradient centrifugation techniques explained.

Protein G and Protein A Bind to Different IgG

This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.

Robust, load independent viral clearance in monoclonal antibody purification with Natrix® Q chromatography membrane

Membrane adsorbers are a cost-effective, robust, single-use solution for monoclonal antibody polishing

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efﬁcient column packing and preparation for affinity chromatography of antibodies.

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.