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Protein Determination by the Warburg-Christian Method
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Protein Determination by the Bicinchoninic Acid (BCA) Method
A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Protein Determination Modified Lowry Method
To standardize a procedure for the determination of protein by modified Lowry.
How to Run Milliplex
Key Steps in the MILLIPLEX® Assay Protocol for the Analysis of Soluble Biomarkers
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Protein Determination using 2-D Quant Kit
2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.
Production of 15N-13C Dual-labelled Proteins
Dr. Alan Robertson and Dr. Ben Davis at Vernalis, Ltd., UK, a provider of drug discovery and development services, have refined a novel protocol for the duallabelling of proteins using EnPresso® B Defined Nitrogen-free.
Enzymatic Method for Determining L-Amino Acids (L-Amino Acid Quantitation Assay)
Assay protocol for the colorimetric/fluorometric detection of L-Amino Acids in biological samples using the L-Amino Acid assay kit
How to Create PLA® Probes
Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.
Duolink® PLA Flow Cytometry Protocol
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.