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Jie Cheng et al.
iScience, 25(1), 103588-103588 (2022-01-11)
HIV-specific T cells have diminished effector function and fail to control/eliminate the virus. IL-27, a member of the IL-6/IL-12 cytokine superfamily has been shown to inhibit HIV replication. However, whether or not IL-27 can enhance HIV-specific T cell function is largely unknown.
Katinka Döhner et al.
Molecular biology of the cell, 13(8), 2795-2809 (2002-08-16)
After fusion of the viral envelope with the plasma membrane, herpes simplex virus type 1 (HSV1) capsids are transported along microtubules (MTs) from the cell periphery to the nucleus. The motor ATPase cytoplasmic dynein and its multisubunit cofactor dynactin mediate
Tao Sun et al.
ACS synthetic biology, 9(7), 1864-1872 (2020-05-30)
Chinese hamster ovary (CHO) cells are the superior host cell culture models used for the bioproduction of therapeutic proteins. One of the prerequisites for bioproduction using CHO cell lines is the need to generate stable CHO cell lines with optimal
Oren Hershkovitz et al.
Journal of immunology (Baltimore, Md. : 1950), 183(4), 2610-2621 (2009-07-29)
Dengue virus (DV) and West Nile virus (WNV) have become a global concern due to their widespread distribution and their ability to cause a variety of human diseases. Antiviral immune defenses involve NK cells. In the present study, we investigated
Jieyi Wang et al.
BMC cancer, 16, 105-105 (2016-02-18)
c-Met is the receptor tyrosine kinase for hepatocyte growth factor (HGF) encoded by the MET proto-oncogene. Aberrant activation of c-Met resulting from MET amplification and c-Met overexpression is associated with poor clinical outcome in multiple malignancies underscoring the importance of
Amy E Arnold et al.
Molecular therapy. Nucleic acids, 11, 518-527 (2018-06-03)
Glioblastoma stem cells (GSCs) are invasive, treatment-resistant brain cancer cells that express downregulated in renal cell carcinoma (DRR), also called FAM107A, a genetic driver of GSC invasion. We developed antibody-antisense oligonucleotide (AON) conjugates to target and reduce DRR/FAM107A expression. Specifically
Yi-Hsin Liang et al.
Scientific reports, 11(1), 9080-9080 (2021-04-29)
Single immunotherapy fails to demonstrate efficacy in patients with microsatellite stable (MSS) metastatic colorectal cancer (mCRC). Research on immune reactions before and after systemic agents for mCRC is warranted. Our study examined cell line models to compare the expression of
Fares Nigim et al.
Targeted oncology, 14(4), 479-489 (2019-07-14)
High-grade meningiomas (HGMs; World Health Organization [WHO] classification grade II and III) have high relapse rates and poor clinical outcomes despite surgery and radiation treatments. No effective medical therapy currently exists for HGMs, and developing novel therapeutic strategies depends on
Latha B Pathangey et al.
Oncotarget, 8(7), 10785-10808 (2016-12-16)
Effective adoptive immunotherapy has proved elusive for many types of human cancer, often due to difficulties achieving robust expansion of natural tumor-specific T-cells from peripheral blood. We hypothesized that antigen-driven T-cell expansion might best be triggered in vitro by acute
Ketil Moen et al.
Clinical and diagnostic laboratory immunology, 10(6), 1043-1050 (2003-11-11)
The objective of the present study was to investigate immunoglobulin G (IgG) and IgA antibody immune responses to Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, and Candida albicans in the sera of patients with rheumatoid arthritis (RA), the synovial fluid (SF)
Stephanie Fischinger et al.
Journal of immunological methods, 473, 112630-112630 (2019-07-14)
The complement system plays a critical role in innate immune defense against pathogens, both via non-specific direct pathogen recognition and killing or via antigen-specific indirect recruitment by complement fixing antibodies. While various assays for measuring complement activation have been developed
CD43 deficiency has no impact in competitive in vivo assays of neutrophil or activated T cell recruitment efficiency.
Carlow, et al.
Journal of Immunology, 177, 6450-6459 (2019)
Ryan L Kelly et al.
mAbs, 7(4), 770-777 (2015-06-06)
Although improvements in technology for the isolation of potential therapeutic antibodies have made the process increasingly predictable, the development of biologically active monoclonal antibodies (mAbs) into drugs can often be impeded by developability issues such as poor expression, solubility, and
Alon D Levin et al.
Journal of Crohn's & colitis, 10(3), 323-329 (2015-09-30)
Anti-tumour necrosis factor [TNF] antibodies induce regulatory macrophages which display a phenotype resembling M2 type macrophages. Anti-TNF induced macrophages [Mϕind] have immunosuppressive and wound healing properties. The factors that contribute to the induction of Mϕind remain to be explored. Autophagy
Haibin Wu et al.
Drug delivery, 24(1), 1216-1229 (2017-08-29)
Since conventional chemotherapy for acute myeloid leukemia (AML) has its limitations, a theranostic platform with targeted and efficient drug transport is in demand. In this study, we developed the first CD123 (AML tumor marker) aptamers and designed a novel CD123-aptamer-mediated
Jeroen F Vermeulen et al.
Molecular imaging and biology, 15(3), 290-298 (2012-11-28)
The purpose of this study was to develop a molecular imaging technique using tracers specific for ductal carcinoma in situ (DCIS) to improve visualization and localization of DCIS during surgery. As CD44v6 is frequently expressed in DCIS, we used near-infrared
Jae Hyeon Park et al.
ACS nano, 14(9), 11950-11961 (2020-08-28)
Silica-coated nanoparticles are widely used in biomedical applications such as theranostics, imaging, and drug delivery. While silica-coated nanoparticles are biocompatible, experimental evidence shows that they can trigger innate immune reactions, and a broader understanding of what types of reactions are
Smita Thobhani et al.
Glycobiology, 19(3), 201-211 (2008-10-14)
As characterization of glycosylation is required for the licensing of recombinant glycoprotein therapeutics, technique comparability must be assessed. Eleven UK laboratories (seven industrial, two regulatory or government, two academic) participated in an inter-laboratory study to analyze N-glycans present in four
Sensitive and fast characterization of site-specific protein glycosylation with capillary electrophoresis coupled to mass spectrometry.
Qu, et al.
Talanta, 179, 22-27 (2019)
Kathirvel Alagesan et al.
Analytical and bioanalytical chemistry, 409(2), 529-538 (2016-12-03)
Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide enrichment efficiency and
Suvajyoti Guha et al.
Journal of pharmaceutical sciences, 101(6), 1985-1994 (2012-03-14)
The biopharmaceutical industry characterizes and quantifies aggregation of protein therapeutics using multiple analytical techniques to cross-validate results. Here, we demonstrate the use of electrospray-differential mobility analysis (ES-DMA), a gas-phase and atmospheric pressure ion-mobility method for characterizing protein aggregates. Two immunoglobulin
Tutku Beduk et al.
Analytical chemistry, 93(24), 8585-8594 (2021-06-04)
The global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has revealed the urgent need for accurate, rapid, and affordable diagnostic tests for epidemic understanding and management by monitoring the population worldwide. Though current diagnostic methods
Danny L Costantini et al.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 51(7), 1084-1091 (2010-06-18)
(111)In-nuclear localization sequence-trastuzumab is a radioimmunotherapeutic agent consisting of trastuzumab modified with NLS peptides (CGYGPKKKRKVGG) and labeled with the Auger electron emitter (111)In. Our objectives were to evaluate the tumor growth-inhibitory properties and normal-tissue toxicity of (111)In-NLS-trastuzumab in mice after
Paul R C Imbert et al.
Current biology : CB, 31(1), 77-89 (2020-10-24)
Macrophages continuously survey their environment in search of pathogens or apoptotic corpses or debris. Targets intended for clearance expose ligands that initiate their phagocytosis ("eat me" signals), while others avoid phagocytosis by displaying inhibitory ligands ("don't eat me" signals). We
Julie L Lucas et al.
PloS one, 12(8), e0182739-e0182739 (2017-08-05)
Cancer therapies can provide substantially improved survival in some patients while other seemingly similar patients receive little or no benefit. Strategies to identify patients likely to respond well to a given therapy could significantly improve health care outcomes by maximizing
B A Castro et al.
Oncogene, 36(26), 3749-3759 (2017-02-22)
Anti-angiogenic therapies for cancer such as VEGF neutralizing antibody bevacizumab have limited durability. While mechanisms of resistance remain undefined, it is likely that acquired resistance to anti-angiogenic therapy will involve alterations of the tumor microenvironment. We confirmed increased tumor-associated macrophages
Tingwan Sun et al.
mAbs, 5(6), 838-841 (2013-09-03)
Self-interaction of an antibody may lead to aggregation, low solubility or high viscosity. Rapid identification of highly developable leads remains challenging, even though progress has been made with the introduction of techniques such as self-interaction chromatography (SIC) and cross-interaction chromatography
Christopher Ashwood et al.
The Analyst, 144(11), 3601-3612 (2019-05-09)
Porous graphitized carbon (PGC) based chromatography achieves high-resolution separation of glycan structures released from glycoproteins. This approach is especially valuable when resolving structurally similar isomers and for discovery of novel and/or sample-specific glycan structures. However, the implementation of PGC-based separations
Shiho Tanaka et al.
Cell reports, 38(6), 110348-110348 (2022-02-04)
The increasing prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with the ability to escape existing humoral protection conferred by previous infection and/or immunization necessitates the discovery of broadly reactive neutralizing antibodies (nAbs). Utilizing mRNA display, we identify
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