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Mohammad Abdul-Moqueet et al.
Nanotechnology (2021-02-20)
Gold bipyramid nanoparticles are promising for a range of biomedical applications, including biosensing and surface-enhanced Raman spectroscopy (SERS), due to their favorable optical properties and ease of chemical functionalization. Here we report improved synthesis methods, including preparation of gold seed
Riccardo Funari et al.
Biosensors & bioelectronics, 169, 112578-112578 (2020-09-11)
The ongoing global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to active research in its associated diagnostics and medical treatments. While quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most reliable method to detect viral
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Jusung Oh et al.
Theranostics, 8(12), 3189-3197 (2018-06-23)
The immunochromatographic (ICA) assay is a highly promising platform for rapid and simple detection of C-reactive protein (CRP) which is an indicator of the different phases of various diseases, as well as of inflammation and infection. However, the hook effect
Orhan Kankavi
Acta biochimica Polonica, 50(4), 1057-1064 (2004-01-24)
The presence of surfactant proteins was investigated in the human organ of Corti, Eustachian tube and kidney tissues. It has previously been shown that lamellar bodies are present in hairy cells of organ of Corti, in the cytoplasm of secretory
Yan-Hong Yan et al.
Acta pharmacologica Sinica, 37(9), 1229-1236 (2016-08-23)
CCL19 and its receptor CCR7 are essential molecules for facilitating the trafficking of mature dendritic cells (DCs) and helping to establish a microenvironment in lymphoid tissues to initiate primary immune responses, whereas CCL17 is required in the CCR7-CCL19-dependent migration of
Govert J van Dam et al.
Experimental parasitology, 135(2), 274-282 (2013-07-16)
An earlier reported laboratory assay, performed in The Netherlands, to diagnose Schistosoma infections by detection of the parasite antigen CAA in serum was converted to a more user-friendly format with dry reagents. The improved assay requires less equipment and allows
Mohsen Rokni et al.
International immunopharmacology, 59, 295-300 (2018-04-21)
Murine hybridoma cells can produce monoclonal antibody (MAb) and the production of these antibodies in culture and peritoneum can be affected by different factors, including stimulants, inhibitors and supplements. Among these factors, the impact of micronutrients on the production of
João G Ribeiro et al.
International immunopharmacology, 47, 227-230 (2017-04-24)
American tegumentary leishmaniasis (ATL) is considered a neglected disease, for which an effective vaccine or an efficient diagnosis is not yet available and whose chemotherapeutic arsenal is threatened by the emergence of resistance by etiological agents such as Leishmania amazonensis.
Yilin Liu et al.
ACS applied nano materials, 4(12), 13826-13837 (2021-12-28)
The SARS-CoV-2 global pandemic created an unprecedented need for rapid, sensitive, and inexpensive point-of-care (POC) diagnostic tests to treat and control the disease. Many POC SARS-CoV-2 lateral flow immunoassays (LFAs) have been developed and/or commercialized, but with only limited sensitivity
Hong Peng et al.
Chemico-biological interactions, 240, 336-345 (2015-09-08)
Human butyrylcholinesterase (HuBChE) is a stoichiometric bioscavenger of nerve agents and organophosphorus pesticides. Mass spectrometry methods detect stable nerve agent adducts on the active site serine of HuBChE. The first step in sample preparation is immunopurification of HuBChE from plasma.
Kidist Bobosha et al.
PLoS neglected tropical diseases, 8(5), e2845-e2845 (2014-05-09)
Field-applicable tests detecting asymptomatic Mycobacterium leprae (M. leprae) infection or predicting progression to leprosy, are urgently required. Since the outcome of M. leprae infection is determined by cellular- and humoral immunity, we aim to develop diagnostic tests detecting pro-/anti-inflammatory and
Jemma Victoria Walker et al.
Nature communications, 10(1), 3596-3596 (2019-08-11)
Stem cells (SCs) receive inductive cues from the surrounding microenvironment and cells. Limited molecular evidence has connected tissue-specific mesenchymal stem cells (MSCs) with mesenchymal transit amplifying cells (MTACs). Using mouse incisor as the model, we discover a population of MSCs
Jin-Hee Han et al.
Journal of biological engineering, 3, 6-6 (2009-04-30)
A microfluidic channel made entirely out of polyethylene glycol (PEG), not PEG coating to silicon or polydimethylsiloxane (PDMS) surface, was fabricated and tested for its reusability in particle immunoassays and passive protein fouling, at relatively high target concentrations (1 mg
Paul L A M Corstjens et al.
Clinical biochemistry, 66, 76-82 (2019-01-30)
New user-friendly diagnostic tests for detection of individuals infected by Mycobacterium leprae (M. leprae), the causative pathogen of leprosy, can help guide therapeutic and prophylactic treatment, thus positively contributing to clinical outcome and reduction of transmission. To facilitate point-of-care testing
Xing-Chen Zhou et al.
The Journal of biological chemistry, 293(8), 2801-2814 (2018-01-07)
The stress-induced unfolded protein response (UPR) in the endoplasmic reticulum (ER) involves various signaling cross-talks and controls cell fate. B-cell receptor (BCR) signaling, which can trigger UPR, induces gammaherpesvirus lytic replication and serves as a physiological mechanism for gammaherpesvirus reactivation
Impaired arterial vitamin D signaling occurs in the development of vascular calcification.
Lim, et al.
PLoS ONE, 15, e0241976-e0241976 (2021)
D R Jayne et al.
Clinical and experimental immunology, 84(3), 476-481 (1991-06-01)
The IgG subclass distribution for both ANCA-specific, and total IgG subclasses, of sera from 48 patients with active systemic vasculitis was determined by solid-phase assay using monoclonal anti-human subclass reagents. To control for varying performance of the subclass reagents, the
Dong Hwan Choi et al.
Biosensors & bioelectronics, 25(8), 1999-2002 (2010-02-20)
For signal amplification without an additional operation step in a gold nanoparticle (AuNP)-based lateral flow assay (LFA), a new and simple method utilizing two AuNP-antibody conjugates was developed. The 1st conjugate was the AuNP immobilized with an anti-troponin I antibody
Jan H Driller et al.
Journal of cell science, 132(6) (2019-02-13)
Protein scaffolds at presynaptic active zone membranes control information transfer at synapses. For scaffold biogenesis and maintenance, scaffold components must be safely transported along axons. A spectrum of kinases has been suggested to control transport of scaffold components, but direct
Anouk van Hooij et al.
Frontiers in immunology, 11, 1811-1811 (2020-08-28)
Leprosy is a chronic infectious disease, caused by Mycobacterium leprae, that can lead to severe life-long disabilities. The transmission of M. leprae is continuously ongoing as witnessed by the stable new case detection rate. The majority of exposed individuals does
V L Esnault et al.
Immunology, 74(4), 714-718 (1991-12-01)
Circulating IgG autoantibodies to myeloperoxidase (MPO) are associated with renal vasculitis and have been implicated in its pathogenesis. However, raised levels of these autoantibodies may persist during clinical remission. We tested whether this paradox could be explained by immunoglobulin subclass
Takumi Watanabe et al.
Optics express, 25(20), 24469-24479 (2017-10-19)
In general, biochemical sensors based on photonic cavities are used to detect changes in the refractive index of the environment. In this study, however, a GaInAsP semiconductor photonic-crystal nanolaser sensor that we recently developed was found to detect not only
K N Lai et al.
Clinical and experimental immunology, 82(2), 233-237 (1990-11-01)
Autoantibodies to neutrophil cytoplasmic antigens are found in patients with small or medium-size vessel systemic vasculitis such as Wegener's granulomatosis and microscopic polyarteritis. We studied the specificity of anti-neutrophil cytoplasm autoantibodies (ANCA) by examining the binding of sera from patients
Mathias Kalxdorf et al.
Nature methods, 18(1), 84-91 (2021-01-06)
Numerous drugs and endogenous ligands bind to cell surface receptors leading to modulation of downstream signaling cascades and frequently to adaptation of the plasma membrane proteome. In-depth analysis of dynamic processes at the cell surface is challenging due to biochemical
Samuel Roulin et al.
Planta, 215(1), 51-59 (2002-05-16)
When seedlings of barley (Hordeum vulgare L.) were transferred from a natural light/dark cycle into darkness, (1-->3,1-->4)-beta- D-glucan endohydrolase (EC 3.2.1.73) activity in leaf extracts increased 3- to 4-fold after 2 days. Activity decreased to normal levels within a day
Ashima Saxena et al.
Archives of biochemistry and biophysics, 652, 38-49 (2018-06-18)
Human butyrylcholinesterase (HuBChE) is under development for use as a pretreatment antidote against nerve agent toxicity. Animals are used to evaluate the efficacy of HuBChE for protection against organophosphorus nerve agents. Pharmacokinetic studies of HuBChE in minipigs showed a mean
E Decroly et al.
FEBS letters, 405(1), 68-72 (1997-03-17)
The intracellular proteolytic processing of HIV envelope glycoprotein gp160 into gp120/gp41 is an essential step for virus infectivity. Several convertases, belonging to the pro-protein convertase family, have been proposed as candidate gp160 processing enzymes. Here we demonstrate using RT-PCR that
Natalie Taylor et al.
Clinical and vaccine immunology : CVI, 19(9), 1416-1425 (2012-07-13)
Mycobacteria have features that make them attractive as potential vaccine vectors. The nonpathogenic and rapidly growing Mycobacterium smegmatis can express both Mycobacterium tuberculosis antigens and heterologous antigens from other pathogens, and it has been used as a viable vector for
Anil B Shrirao et al.
Biofabrication, 6(3), 035016-035016 (2014-07-06)
Substrate and cell patterning are widely used techniques in cell biology to study cell-to-cell and cell-substrate interactions. Conventional patterning techniques work well only with simple shapes, small areas and selected bio-materials. This paper describes a method to distribute cell suspensions
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