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P1378

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Urszula Posadowska et al.
Acta of bioengineering and biomechanics, 17(3), 41-48 (2015-12-22)
Since there are more and more cases of multiresistance among microorganisms, rational use of antibiotics (especially their systemic vs. local application) is of great importance. Here we propose polymeric nanoparticles as locally applied gentamicin delivery system useful in osteomyelitis therapy....
A Vasanits-Zsigrai et al.
Journal of chromatography. A, 1394, 81-88 (2015-04-05)
Quantification, stability and unique spectroscopic properties of indolinyl-caged glutamates (ICGs), with the o-phthalaldehyde-3-mercaptopropionic acid (OPA-MPA) reagent, were described, at first. As new principle to the field, reactivity and stoichiometry of variously substituted OPA-MPA derivatized ICGs, such as 4-methoxy-7-nitroindolinyl-(MNI-Glu), 4-methoxy-5,7-dinitroindolinyl-(DNI-Glu), 2-dimethylamino-propoxy...
Stéphane Mann et al.
Analytical biochemistry, 432(2), 90-96 (2012-10-17)
7,8-Diaminopelargonic acid (DAPA) aminotransferase is an enzyme of the biotin biosynthetic pathway that plays an essential role in Mycobacterium tuberculosis virulence. Inhibition of this enzyme is a potential strategy to combat this microorganism, the causative agent of tuberculosis. To identify...
James McMasters et al.
The AAPS journal, 17(5), 1117-1125 (2015-06-14)
Peripheral artery disease is an atherosclerotic occlusion in the peripheral vasculature that is typically treated via percutaneous transluminal angioplasty. Unfortunately, deployment of the angioplasty balloon damages the endothelial layer, exposing the underlying collagen and allowing for the binding and activation...
Yi-Jhen Lai et al.
Talanta, 91, 103-109 (2012-03-01)
This study reports a selective and sensitive method for fluorescent detection of total, protein-bound, free, and free oxidized homocysteine (HCys) using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent, fluorosurfactant-capped gold nanoparticles (FSN-AuNP) as a preconcentrating probe, and o-phthaldialdehyde (OPA) as a...
Qingyuan Dai et al.
Journal of agricultural and food chemistry, 63(16), 4179-4189 (2015-04-07)
A simple and green method was developed for preparing the stable biopolymer nanoparticles with pH and salt resistance. The method involved the macromolecular crowding Maillard process and heat-induced gelation process. The conjugates of whey protein isolate (WPI) and dextran were...
Tiberiu Popescu et al.
Life sciences, 117(2), 75-83 (2014-10-07)
The study investigated the effects of the combined treatment Parecoxib (Pcox) and 5,10,15,20-tetra-sulphonato-phenyl-porphyrin(TSPP)-mediated photodynamic therapy on Walker 256 carcinosarcoma. Five groups of male Wistar rats were used: the control group, treated with TSPP, group 2, irradiated 24 h thereafter, group...
Wei-Yao Chang et al.
Journal of chromatography. A, 1248, 41-47 (2012-06-26)
A new one-step derivatization and microextraction technique was developed for the fluorometric determination of C(1)-C(8) linear aliphatic primary amines in complex sample solutions containing high levels of amino acids. In this method, amines were derivatized with o-phthalaldehyde (OPA) and 2-mercaptoethanol...
Yanier Nuñez-Figueredo et al.
Neurochemistry international, 81, 41-47 (2015-01-27)
JM-20 (3-ethoxycarbonyl-2-methyl-4-(2-nitrophenyl)-4,11-dihydro-1H-pyrido[2,3-b][1,5]benzodiazepine) is a novel benzodiazepine dihydropyridine hybrid molecule, which has been shown to be a neuroprotective agent in brain disorders involving glutamate receptors. However, the effect of JM-20 on the functionality of the glutamatergic system has not been investigated....
Yuchen Guo et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-28)
Pterygoplichthys disjunctivus, locally named the armoured catfish, is a by-catch of tilapia fishing that accounts for up to 80% of total captured fish in the Adolfo Lopez Mateos dam, in Michoacán, México, affecting the economy of its surrounding communities. This...
Saravana Periaswamy Sivagnanam et al.
Marine drugs, 13(6), 3422-3442 (2015-06-04)
The bioactive materials in brown seaweeds hold great interest for developing new drugs and healthy foods. The oil content in brown seaweeds (Saccharina japonica and Sargassum horneri) was extracted by using environmentally friendly supercritical CO2 (SC-CO2) with ethanol as a...
Martina Sandberg Abelius et al.
Journal of reproductive immunology, 106, 100-109 (2014-07-23)
The immunological milieu in the placenta may be crucial for priming the developing foetal immune system. Early imbalances may promote the establishment of immune-mediated diseases in later life, including allergies. The initial exposure to allergens seems to occur in utero...
Alphonse Mendy et al.
Journal of fluorescence, 29(3), 515-522 (2019-06-06)
Improvement of the TIFD method to determine buprofezin (BUP) founded on the O-phthalaldehyde (OPA)-thermoproduct complex of buprofezin (OPA-BUP) with dimethyl-β-cyclodextrin (DMβCD) was investigated at 25 °C. The TIFD method developed in DMβCD medium, was optimized with respect to the reaction time...
Chamila Nimalaratne et al.
Food chemistry, 194, 111-116 (2015-10-17)
The aromatic amino acids and carotenoids are the major contributors to the antioxidant properties of egg yolk. This study aimed to evaluate the effect of simulated retail storage and domestic cooking on the antioxidant activity as well as on the...
I Molnár-Perl
Journal of chromatography. A, 913(1-2), 283-302 (2001-05-18)
An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have...
Jessica Murphy et al.
Experimental gerontology, 69, 20-26 (2015-06-13)
Obesity and type 2 diabetes have been shown to alter the insulin sensitivity of glucose and protein metabolism in middle-aged women. We aimed to determine whether these findings translate to the elderly who are at increased risk of muscle loss....
Yunfei Tu et al.
Se pu = Chinese journal of chromatography, 30(2), 184-189 (2012-06-12)
A method of precolumn derivatization-high performance liquid chromatography (HPLC) for the determination of theanine and gamma-aminobutyric acid (GABA) in tea was established. o-Phthalaldehyde (OPA) and N-acetyl-L-cysteine (NAC) were chosen as the derivatization reagents. The effects of teapolyphenol (Tp), proline (Pro)...
Atsuo Iwasawa et al.
Biocontrol science, 16(4), 165-170 (2011-12-23)
We investigated the bactericidal effects and cytotoxicity of an ortho-phthalaldehyde product in comparison with those of its predecessor glutaraldehyde products. Bactericidal effects ware examined on Mycobacterium terrae, a standard organism used for investigating the bactericidal effect of high-level disinfectants. Cytotoxicity...
Giuseppe Carlucci et al.
Biomedical chromatography : BMC, 29(6), 911-917 (2014-10-31)
A simple and sensitive method based on the combination of derivatization and high-performance liquid chromatography with ultraviolet and fluorimetric detection was developed for the simultaneous determination of octreotide and gabexate mesylate metabolite in human pancreatic juice samples. Parameters of the...
Tidjani Amza et al.
Journal of food science and technology, 52(5), 2770-2778 (2015-04-22)
The antioxidant capacity of gingerbread plum kernel protein fraction (albumin, globulin and glutelin) hydrolysates (GPKH) was studied. Gingerbread plum kernel protein fractions were hydrolyzed through a combined action of two digestive enzymes (pespsin and trypsin). The hydrolyzed fractions were subjected...
Peter J Gilling et al.
BJU international, 112 Suppl 2, 69-73 (2013-10-30)
To compare the effectiveness, safety and cost of Tristel Fuse (chlorine dioxide) with Cidex OPA (ortho-phthaldehyde; 1,2-benzenedicarboxaldehyde) in an automated endoscopic reprocessor (AER) for high-level disinfection of flexible cystoscopes. A randomised single-blind study comparing the high-level disinfectants Tristel Fuse as...
Benjamin D Bradford et al.
JAMA otolaryngology-- head & neck surgery, 139(6), 574-578 (2013-05-18)
If not adequately cleaned, rigid nasal endoscopes (RNEs) have the potential to cause iatrogenic cross-contamination. To test the efficacy of various disinfection methods in reducing bacterial load on RNEs in vitro. In vitro model. Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa...
Jin-Aa Oh et al.
Journal of chromatography. A, 1247, 99-103 (2012-06-12)
A simple high performance liquid chromatographic (HPLC) method and a highly sensitive gas chromatography mass spectrometric (GC-MS) method have been established for the determination of ortho-phthalaldehyde (OPA) in water. These methods are based on the derivatization of OPA with hydrazine...
Shaoxuan Yu et al.
Nanotechnology, 26(14), 145703-145703 (2015-03-19)
Selenium nanoparticles (Se NPs) possess well-known excellent biological activities and low toxicity, and have been employed for numerous applications except as inhibitors to protein glycation. Herein, the present study is carried out to investigate the inhibitory effect of Se NPs...
Mohammed Nadeem Bijle et al.
Journal of dentistry, 99, 103411-103411 (2020-06-26)
To examine the effect of incorporating arginine in a 5% sodium fluoride (NaF) varnish on its remineralization potential and HGF-1 cytotoxicity. Experimental varnishes were prepared using two arginine variants: l-arginine (Arg) and l-arginine monohydrochloride (Arg.HCl) at 2%, 4%, & 8%...
Zoltan Nemeth et al.
PloS one, 10(8), e0135498-e0135498 (2015-08-28)
Pericardial fluid (PF) contains several biologically active substances, which may provide information regarding the cardiac conditions. Nitric oxide (NO) has been implicated in cardiac remodeling. We hypothesized that L-arginine (L-Arg) precursor of NO-synthase (NOS) and asymmetric dimethylarginine (ADMA), an inhibitor...
Tomáš Roušar et al.
Analytical biochemistry, 423(2), 236-240 (2012-03-06)
Glutathione is an important tripeptide involved in a variety of cellular processes. Thus, precise knowledge of its levels is essential. Glutathione exists in two free forms-reduced and oxidized-and a number of methods exist to measure its levels. The aim of...
Katsuhiro Suzuki et al.
Biomedical chromatography : BMC, 27(4), 535-538 (2012-09-29)
Monomethylarginine, asymmetric dimethylarginine and symmetric dimethylarginine were separated on a Wakopak Combi ODS with an acetonitrile-100 mm potassium phosphate buffer (pH 7.0; 1:1, v/v). Dimethylarginines were derived from o-phthalaldehyde for the fluorescence detector and from 6-ferrocenyl-1-hexanethiol for the electrochemical detector. The...
Cristina Bruno et al.
Journal of immunology (Baltimore, Md. : 1950), 195(1), 166-173 (2015-05-29)
The generation of CTLs is crucial in the immunological fight against cancer and many infectious diseases. To achieve this, vaccine Ags need to be targeted to the cytosol of dendritic cells, which can activate CD8 T cells via MHC class...
Estefanía de Munck et al.
Environmental toxicology and pharmacology, 39(3), 1232-1245 (2015-05-24)
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive muscle paralysis that reflects the motoneurons' degeneration. Several studies support the relationship between β-N-methylamino-l-alanine (l-BMAA), a neurotoxic amino acid produced by cyanobacteria and diatoms, and the sporadic occurrence of...
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