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Kyung-Hyun Cho
Molecules (Basel, Switzerland), 26(14) (2021-07-25)
Beta (β)-amyloid (Aβ) is a causative protein of Alzheimer's disease (AD). In the pathogenesis of AD, the apolipoprotein (apo) A-I and high-density lipoprotein (HDL) metabolism is essential for the clearance of Aβ. In this study, recombinant Aβ42 was expressed and...
Nancy Meyer et al.
Bio-protocol, 10(3), e3513-e3513 (2021-03-04)
Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for the production of: (a) the...
Nicholas C Wu et al.
Cell reports, 33(3), 108274-108274 (2020-10-08)
IGHV3-53-encoded neutralizing antibodies are commonly elicited during SARS-CoV-2 infection and target the receptor-binding domain (RBD) of the spike (S) protein. Such IGHV3-53 antibodies generally have a short CDR H3 because of structural constraints in binding the RBD (mode A). However...
L V Albrecht et al.
STAR protocols, 1(3), 100132-100132 (2020-12-31)
Lysosomes are the catabolic center of the cell. Limitations of many lysosomal tracers include low specificity and lack of reliable physiological readouts for changes in growth factor-regulated lysosomal activity. The imaging-based protocols described here provide insights at the cellular level...
Xiaojun Lu et al.
mAbs, 11(1), 45-57 (2018-12-12)
Contemporary in vivo and in vitro discovery platform technologies greatly increase the odds of identifying high-affinity monoclonal antibodies (mAbs) towards essentially any desired biologically relevant epitope. Lagging discovery throughput is the ability to select for highly developable mAbs with drug-like...
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Marion Poirier et al.
ChemMedChem, 14(2), 224-236 (2018-12-07)
By screening a focused library of kinase inhibitor analogues in a phenotypic co-culture assay for angiogenesis inhibition, we identified an aminotriazine that acts as a cytostatic nanomolar inhibitor. However, this aminotriazine was found to be completely inactive in a whole-kinome...
Georg Hafner et al.
Cell reports, 28(13), 3450-3461 (2019-09-26)
Parvalbumin (PV)-expressing GABAergic neurons are the largest class of inhibitory neocortical cells. We visualize brain-wide, monosynaptic inputs to PV neurons in mouse barrel cortex. We develop intersectional rabies virus tracing to specifically target GABAergic PV cells and exclude a small...
Zuriñe Antón et al.
Journal of cell science, 133(14) (2020-06-10)
The sorting nexins (SNXs) are a family of peripheral membrane proteins that direct protein trafficking decisions within the endocytic network. Emerging evidence in yeast and mammalian cells implicates a subgroup of SNXs in selective and non-selective forms of autophagy. Using...
Luke W Vanderheyden et al.
Journal of applied physiology (Bethesda, Md. : 1985), 128(5), 1321-1328 (2020-04-03)
High-intensity exercise inhibits appetite, in part, via alterations in the peripheral concentrations of the appetite-regulating hormones acylated ghrelin, active glucagon-like peptide-1 (GLP-1), and active peptide tyrosine-tyrosine (PYY). Given lactate may mediate these effects, we used sodium bicarbonate (NaHCO3) supplementation in...
Christopher S Hale et al.
The Review of scientific instruments, 90(3), 034102-034102 (2019-04-01)
The membrane concentration osmometer coupled with multiple sample preparations has been used for over a century to determine a number of colloidal properties. At the dilute region, this method has been used to determine solute molecular mass. When the solution...
Martin G Klatt et al.
Journal of proteomics, 228, 103921-103921 (2020-08-08)
Characterization of MHC-bound peptides by mass spectrometry (MS) is an essential technique for immunologic studies. Many efforts have been made to quantify the number of MHC-presented ligands by MS and to define the limits of detection of a specific MHC...
Louise Thines et al.
Bio-protocol, 10(5), e3542-e3542 (2021-03-05)
Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2 -) into molecular oxygen (O2) and hydrogen peroxide (H2O2). Members of this enzyme family include CuZnSODs, MnSODs, FeSODs, and NiSODs, depending...
Bhuvaneshwari Manivannan et al.
Ecotoxicology and environmental safety, 203, 110984-110984 (2020-09-06)
The use of water for drinking and agriculture requires knowledge of its toxicity. In this study, we compared the use of genetically modified bioluminescent (GMB) bacteria whose luminescence increases in the presence of toxicants and Chinese Hamster Ovary (CHO) cells...
D T Gruszka et al.
Science advances, 6(38) (2020-09-20)
During replication, nucleosomes are disrupted ahead of the replication fork, followed by their reassembly on daughter strands from the pool of recycled parental and new histones. However, because no previous studies have managed to capture the moment that replication forks...
Stefanos Stagkourakis et al.
Cell, 182(4), 960-975 (2020-08-09)
Parental behavior is pervasive throughout the animal kingdom and essential for species survival. However, the relative contribution of the father to offspring care differs markedly across animals, even between related species. The mechanisms that organize and control paternal behavior remain...
Megan Davis-Fields et al.
Biophysical journal, 117(8), 1496-1507 (2019-10-07)
Biofilm infections can consist of bacterial aggregates that are an order of magnitude larger than neutrophils, phagocytic immune cells that densely surround aggregates but do not enter them. Because a neutrophil is too small to engulf the entire aggregate, it...
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Fei Wang et al.
Current biology : CB, 30(19), 3749-3760 (2020-08-17)
The mating decisions of Drosophila melanogaster females are primarily revealed through either of two discrete actions: opening of the vaginal plates to allow copulation, or extrusion of the ovipositor to reject the male. Both actions are triggered by the male...
Sungchul Kim et al.
Nature, 579(7797), 141-145 (2020-02-23)
CRISPR-Cas immunity protects prokaryotes against invading genetic elements1. It uses the highly conserved Cas1-Cas2 complex to establish inheritable memory (spacers)2-5. How Cas1-Cas2 acquires spacers from foreign DNA fragments (prespacers) and integrates them into the CRISPR locus in the correct orientation...
Bianca Johansen et al.
Antibiotics (Basel, Switzerland), 9(7) (2020-07-12)
Tea Tree oil (TTO) is well known for its numerous good properties but might be also irritating or toxic when used topically or ingested, thus limiting the number of possible applications in Humans. The aim of the study was to...
Noemi Holderith et al.
Cell reports, 32(4), 107968-107968 (2020-07-30)
Elucidating the molecular mechanisms underlying the functional diversity of synapses requires a high-resolution, sensitive, diffusion-free, quantitative localization method that allows the determination of many proteins in functionally characterized individual synapses. Array tomography permits the quantitative analysis of single synapses but...
Sona Vodenkova et al.
Nature protocols, 15(12), 3844-3878 (2020-11-18)
This optimized protocol (including links to instruction videos) describes a comet-based in vitro DNA repair assay that is relatively simple, versatile, and inexpensive, enabling the detection of base and nucleotide excision repair activity. Protein extracts from samples are incubated with...
Alexey Veremeev et al.
Materials (Basel, Switzerland), 13(15) (2020-08-06)
Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized...
Linda Kvastad et al.
Communications biology, 4(1), 57-57 (2021-01-10)
The RNA integrity number (RIN) is a frequently used quality metric to assess the completeness of rRNA, as a proxy for the corresponding mRNA in a tissue. Current methods operate at bulk resolution and provide a single average estimate for...
Pengfei Wang et al.
Cell host & microbe, 29(5), 747-751 (2021-04-23)
The emergence of SARS-CoV-2 variants has raised concerns about altered sensitivity to antibody-mediated immunity. The relative resistance of SARS-CoV-2 variants B.1.1.7 and B.1.351 to antibody neutralization has been recently investigated. We report that another emergent variant from Brazil, P.1, is...
Yehou M D Gnopo et al.
Journal of colloid and interface science, 578, 522-532 (2020-06-17)
Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles. The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations. To date there are no reports of induced fusion of bacterial...
Nicholas C Wu et al.
Cell host & microbe, 28(3), 434-444 (2020-07-04)
Understanding how broadly neutralizing antibodies (bnAbs) to influenza hemagglutinin (HA) naturally develop in humans is critical to the design of universal influenza vaccines. Several classes of bnAbs directed to the conserved HA stem were found in multiple individuals, including one...
Michael G Mason et al.
Nature protocols, 15(11), 3663-3677 (2020-10-03)
The complexity of current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick....
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