Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.

Molecular and cellular biology (2005-02-03)
Petra Steinacker, Petra Schwarz, Kerstin Reim, Peter Brechlin, Olaf Jahn, Hartmut Kratzin, Alastair Aitken, Jens Wiltfang, Adriano Aguzzi, Erik Bahn, Helen C Baxter, Nils Brose, Markus Otto
RESUMEN

The diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) is based on typical clinical findings and is supported by a positive 14-3-3 Western blot of cerebrospinal fluid. However, it is not clear whether 14-3-3 indicates general neuronal damage or is of pathophysiological relevance in CJD. The fact that the 14-3-3 isoform spectrum in cerebrospinal fluid does not correspond to that found in the brain points to a regulated process. To investigate a possible role of 14-3-3 proteins in transmissible spongiform diseases, we generated a 14-3-3gamma-deficient mutant mouse line by using a classical knockout strategy. The anatomy and cage behavior of the mutant mice were normal. Western blot analyses of brain homogenates revealed no changes in the protein expression of other 14-3-3 isoforms (epsilon, beta, zeta, and eta). Proteomic analyses of mouse brains by two-dimensional differential gel electrophoresis showed that several proteins, including growth hormone, 1-Cys peroxiredoxin, CCT-zeta, glucose-6-phosphate isomerase, GRP170 precursor, and alpha-SNAP, were differentially expressed. Mutant and wild-type mice were inoculated either intracerebrally or intraperitoneally with the Rocky Mountain Laboratory strain of scrapie, but no differences were detected in the postinoculation survival rates. These results indicate that 14-3-3gamma is unlikely to play a causal role in CJD and related diseases.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Anti-14-3-3 β Antibody, serum, Chemicon®
Sigma-Aldrich
Anti-14-3-3 η Antibody, serum, Chemicon®
Sigma-Aldrich
Anti-14-3-3 ε Antibody, CT, serum, Chemicon®
Sigma-Aldrich
Anti-14-3-3 γ Antibody, serum, Chemicon®
Sigma-Aldrich
Anti-14-3-3 ζ Antibody, serum, Chemicon®

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.