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Synthesis and bioconjugation of alkanethiol-stabilized gold bipyramid nanoparticles.

Nanotechnology (2021-02-20)
Mohammad Abdul-Moqueet, Leeana Tovias, Priscilla Lopez, Kathryn M Mayer
ABSTRACT

Gold bipyramid nanoparticles are promising for a range of biomedical applications, including biosensing and surface-enhanced Raman spectroscopy (SERS), due to their favorable optical properties and ease of chemical functionalization. Here we report improved synthesis methods, including preparation of gold seed particles with an increased shelf life of ~1 month, and preparation of gold bipyramids with significantly shortened synthesis time (< 1 hour). We also report methods for the functionalization and bioconjugation of the gold bipyramids, including functionalization with alkanethiol self-assembled monolayers (SAMs) and bioconjugation with proteins via carbodiimide cross-linking. Binding of specific antibodies to the nanoparticle-bound proteins was subsequently observed via localized surface plasmon resonance (LSPR) sensing. Rabbit IgG and goat anti-Rabbit IgG antibodies were used as a model system for antibody-antigen interactions. As-synthesized, SAM-functionalized, and bioconjugated bipyramids were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, zeta potential, and dynamic light scattering (DLS).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Mouse IgG (whole molecule) antibody produced in goat, affinity isolated antibody, lyophilized powder
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule) antibody produced in goat, affinity isolated antibody, lyophilized powder