• Inicio
  • Resultados de la búsqueda
  • In vitro antioxidant and antimicrobial activities of Merremia emarginata using thio glycolic acid-capped cadmium telluride quantum dots.

In vitro antioxidant and antimicrobial activities of Merremia emarginata using thio glycolic acid-capped cadmium telluride quantum dots.

Colloids and surfaces. B, Biointerfaces (2012-07-17)
A Rameshkumar, T Sivasudha, R Jeyadevi, B Sangeetha, D Arul Ananth, G Smilin Bell Aseervatham, N Nagarajan, R Renganathan, A Kathiravan
RESUMEN

This study was undertaken to evaluate the antioxidant potential of an aqueous extract from Merremia emarginata leaves because this plant has a very high flavonoid and phenol content. The in vitro antioxidant activity was measured by diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS), superoxide anion scavenging assay and lipid peroxidation activity; the total reducing capability of the plant extract indicates that this plant is a source for natural antioxidants. Furthermore, we investigated thio glycolic acid-capped cadmium telluride quantum dots (TGA-CdTe QDs) as fluorescent probes to study the antioxidant activity of the M. emarginata extract through fluorescence quenching. The antimicrobial activity was also investigated using a disc diffusion method and fluorescence microscopy. The TGA-CdTe QDs and M. emarginata complex could provide antimicrobial activity through a reactive oxygen species pathway and/or microbial endocytosis through an electrostatic attraction. Based on our findings, we suggest that the QDs act as potential probes for the in vitro antioxidant and antimicrobial activities. In addition, their cooperative effect with the plant extract indicates that QDs could be used as nanocarriers to enhance the antimicrobial capability. Further in vivo studies on the photolabelling of antioxidants with QDs will provide insights into the mechanistic pathways of secondary metabolites against various degenerative diseases.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Thioglycolic acid, 98%
Sigma-Aldrich
Sodium thioglycolate, ≥96.5% (iodometric)
Sigma-Aldrich
Thioglycolic acid, ≥99%
Sigma-Aldrich
CdTe core-type quantum dots, COOH functionalized, fluorescence λem 610 nm, powder
Sigma-Aldrich
Thioglycolic acid solution, ~70 % (w/w) in H2O
Supelco
Thioglycolic acid solution, ~80% in H2O, for spectrophotometric det. of palladium, iron, uranium(VI), molybdates and nitrites
Sigma-Aldrich
CdTe core-type quantum dots, COOH functionalized, fluorescence λem 520 nm, powder
Sigma-Aldrich
CdTe core-type quantum dots, COOH functionalized, fluorescence λem 710 nm, powder
Sigma-Aldrich
CdTe core-type quantum dots, COOH functionalized, fluorescence λem 570 nm, powder
Sigma-Aldrich
CdTe core-type quantum dots, COOH functionalized, fluorescence λem 770 nm, powder
Sigma-Aldrich
CdTe core-type quantum dots, COOH functionalized, fluorescence λem 510 nm, powder

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.