Merck
  • Home
  • Search Results
  • Contribution of 3-hydroxyisobutyrate to the measurement of 3-hydroxybutyrate in human plasma: comparison of enzymatic and gas-liquid chromatography-mass spectrometry assays in normal and in diabetic subjects.

Contribution of 3-hydroxyisobutyrate to the measurement of 3-hydroxybutyrate in human plasma: comparison of enzymatic and gas-liquid chromatography-mass spectrometry assays in normal and in diabetic subjects.

Journal of lipid research (1989-11-01)
A Avogaro, D M Bier
ABSTRACT

In this study we employed a capillary gas-liquid chromatographic-mass spectrometric (GLC-MS) method to measure the plasma concentrations of 3-hydroxybutyrate (3-OHB) and 3-hydroxyisobutyrate (3-OHIB) in overnight fasted diabetic subjects and in normal subjects. Plasma contents of 3-hydroxybutyrate measured in this fashion were identical to those obtained by enzymatic assay using a commercial preparation of beta-hydroxybutyrate dehydrogenase, indicating no significant contamination of this enzyme preparation with 3-hydroxyisobutyrate dehydrogenase. In normal individuals, plasma 3-OHIB concentration was 21 +/- 2 microM in the overnight fasted state and was higher in diabetic subjects (38 +/- 5 microM) and in subjects fasted for 72 h (97 +/- 4 microM). In the postabsorptive state, 3-OHIB was 33% the concentration of 3-OHB in normals and 17% that of 3-OHB in the diabetics.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glycerol, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
Sigma-Aldrich
Glycerol, for molecular biology, ≥99.0%
Sigma-Aldrich
L-Carnitine inner salt, synthetic, ≥98%
Sigma-Aldrich
Methylglyoxal solution, ~40% in H2O
Sigma-Aldrich
Estriol, ≥97%
Sigma-Aldrich
Estriol, meets USP testing specifications
Sigma-Aldrich
1-Methyl-L-histidine, ≥98.0% (TLC)
Sigma-Aldrich
(R)-3-Hydroxybutyric acid, ≥98.0% (T)
Sigma-Aldrich
(S)-3-Hydroxybutyric acid, ≥97.0%
Sigma-Aldrich
Glycerol, BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
Sigma-Aldrich
Methylglyoxal solution, technical, ~40% in H2O
Sigma-Aldrich
Dimethylamine solution, purum, 33% in absolute ethanol (~5.6 M)
Sigma-Aldrich
Glycerol, tested according to Ph. Eur., anhydrous
Supelco
4-Heptanone, analytical standard
Sigma-Aldrich
3-Methyl-L-histidine
Supelco
Estriol, VETRANAL®, analytical standard
Sigma-Aldrich
Glycerol, ACS reagent, ≥99.5%
Sigma-Aldrich
Glycerol, ReagentPlus®, ≥99.0% (GC)
Sigma-Aldrich
4-Heptanone, 98%
Sigma-Aldrich
4-Heptanone, ≥97%, FG
Sigma-Aldrich
Dimethylamine solution, 2.0 M in THF
Sigma-Aldrich
Dimethylamine solution, 2.0 M in methanol
Sigma-Aldrich
Butyl alcohol, natural, ≥99.5%, FCC, FG
Sigma-Aldrich
Glycerol, FCC, FG
Supelco
Acetone solution, certified reference material, 2000 μg/mL in methanol: water (9:1)
Supelco
Estriol solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Levocarnitine, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Glycerol, BioXtra, ≥99% (GC)
Sigma-Aldrich
Glycerol, ≥99.5%
Sigma-Aldrich
Dimethylamine solution, 40 wt. % in H2O