Merck
  • Home
  • Search Results
  • Molecularly imprinted protein recognition thin films constructed by controlled/living radical polymerization.

Molecularly imprinted protein recognition thin films constructed by controlled/living radical polymerization.

Journal of bioscience and bioengineering (2014-07-26)
Shogo Sasaki, Tooru Ooya, Yukiya Kitayama, Toshifumi Takeuchi
ABSTRACT

We demonstrated the synthesis of molecularly imprinted polymers (MIPs) with binding affinity toward a target protein, ribonuclease A (RNase) by atom transfer radical polymerization (ATRP) of acrylic acid, acrylamide, and N,N'-methylenebisacrylamide in the presence of RNase. The binding activity of the MIPs was evaluated by surface plasmon resonance (SPR) of the MIP thin layers prepared on the gold-coated sensor chips. The MIPs prepared by ATRP (MIP-ATRP) had a binding affinity toward RNase with larger binding amount compared to MIPs prepared by conventional free radical polymerization methods (MIP-RP). Moreover, protein selectivity was evaluated using reference proteins (cytochrome c, myoglobin, and α-lactalbumin) and was confirmed in MIP-ATRP of optimum film thickness determined experimentally to be 15-30 nm; however, protein selectivity was not achieved in all MIP-RP. We have shown that ATRP is powerful technique for preparing protein recognition materials by molecular imprinting.

MATERIALS
Product Number
Brand
Product Description

Supelco
Dichloromethane, analytical standard
Supelco
Ethanol, standard for GC
Supelco
Dichloromethane, Selectophore, ≥99.5%
Sigma-Aldrich
Ethanol, purum, secunda spirit, denaturated with 2% 2-butanone and 0.5% 4-methyl-2-pentanone, S15, ~96% (based on denaturant-free substance)
Sigma-Aldrich
Ethanol, tested according to Ph. Eur.
Sigma-Aldrich
Ethanol, for residue analysis
Sigma-Aldrich
Sodium hydroxide-16O solution, 20 wt. % in H216O, 99.9 atom % 16O
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Sigma-Aldrich
N,N,N′,N′′,N′′-Pentamethyldiethylenetriamine, 99%
Sigma-Aldrich
1,2-Dichloroethane, anhydrous, 99.8%
Sigma-Aldrich
N-Hydroxysuccinimide, 98%
Supelco
Dichloromethane solution, certified reference material, 5000 μg/mL in methanol
Supelco
Dichloromethane solution, certified reference material, 200 μg/mL in methanol
Sigma-Aldrich
4-(Dimethylamino)pyridine, purum, ≥98.0% (NT)
Sigma-Aldrich
Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
N-Hydroxysuccinimide, purum, ≥97.0% (T)
Supelco
1,2-Dichloroethane, analytical standard
Supelco
Ascorbic Acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
4-(Dimethylamino)pyridine, ReagentPlus®, ≥99%
USP
Ascorbic acid, United States Pharmacopeia (USP) Reference Standard
Supelco
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 5 mm × 8 in.
Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Supelco
Methanol solution, suitable for HPLC, contains 10 % (v/v) water, 0.1 % (v/v) trifluoroacetic acid
Sigma-Aldrich
Ethanol, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)
Ascorbic acid, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
1,2-Dichloroethane, suitable for HPLC, ≥99.8%
Sigma-Aldrich
Dichloromethane, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, ACS reagent, meets USP testing specifications, Excise Tax-free, Permit for use required
Sigma-Aldrich
Dichloromethane, suitable for HPLC, ≥99.8%, contains amylene as stabilizer
Sigma-Aldrich
1,2-Dichloroethane, ACS reagent, ≥99.0%