Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons (2014-06-18)
B Gao, C Moore, F Porcheray, C Rong, C Abidoglu, J DeVito, R Paine, T C Girouard, S L Saidman, D Schoenfeld, B Levin, W Wong, N Elias, C Schuetz, I Rosales, Y Fu, E Zorn
RESUMEN

Preexisting serum antibodies have long been associated with graft loss in transplant recipients. While most studies have focused on HLA-specific antibodies, the contribution of non-HLA-reactive antibodies has been largely overlooked. We have recently characterized mAbs secreted by B cell clones derived from kidney allograft recipients with rejection that bind to apoptotic cells. Here, we assessed the presence of such antibodies in pretransplant serum from 300 kidney transplant recipients and examined their contribution to the graft outcomes. Kaplan-Meier survival analysis revealed that patients with high pretransplant IgG reactivity to apoptotic cells had a significantly increased rate of late graft loss. The effect was only apparent after approximately 1 year posttransplant. Moreover, the association between pretransplant IgG reactivity to apoptotic cells and graft loss was still significant after excluding patients with high reactivity to HLA. This reactivity was almost exclusively mediated by IgG1 and IgG3 with complement fixing and activating properties. Overall, our findings support the view that IgG reactive to apoptotic cells contribute to presensitization. Taking these antibodies into consideration alongside anti-HLA antibodies during candidate evaluation would likely improve the transplant risk assessment.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Human IgG ELISA Kit, for serum, plasma
Sigma-Aldrich
Anti-Fas Antibody (human, activating), clone CH11, clone CH11, Upstate®, from mouse
Sigma-Aldrich
Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, clone GG-7, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, clone HP-6017, ascites fluid
Sigma-Aldrich
Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal, clone HP-6017, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal antibody produced in mouse, clone GT-34, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Rabbit IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in mouse, clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal, clone RG-96, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Rabbit IgG (γ-chain specific) antibody produced in mouse, clone RG-96, ascites fluid
Sigma-Aldrich
Anti-Human IgG (Fc specific)−FITC antibody, Mouse monoclonal antibody produced in mouse, clone HP-6017, purified from hybridoma cell culture
Sigma-Aldrich
Mouse IgG2a ELISA Kit
Sigma-Aldrich
Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse, clone GT-34, ascites fluid
Sigma-Aldrich
Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal antibody produced in mouse, clone RabT-50, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse, clone GG-6, ascites fluid
Sigma-Aldrich
Anti-Rabbit IgG, Native antibody, Mouse monoclonal, clone RabT-50, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal, clone RG-96, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Human IgG2 (Fc specific) antibody produced in mouse, clone HP-6002, ascites fluid
Sigma-Aldrich
Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal, clone GT-34, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Goat/Sheep IgG−Alkaline Phosphatase antibody, Mouse monoclonal, clone GT-34, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Goat/Sheep IgG−FITC antibody, Mouse monoclonal antibody produced in mouse, clone GT-34, purified from hybridoma cell culture
Sigma-Aldrich
Mouse IgG3 ELISA Kit
Sigma-Aldrich
Monoclonal Anti-Human IgG4 (pFc′ specific) antibody produced in mouse, clone HP-6023, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse, clone GG-5, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Rat IgG2a antibody produced in mouse, clone R2A-2, ascites fluid