Solid-phase synthesis of short α-helices stabilized by the hydrogen bond surrogate approach.

Nature protocols (2010-10-30)
Anupam Patgiri, Monica Z Menzenski, Andrew B Mahon, Paramjit S Arora
RESUMEN

Stabilized α-helices and nonpeptidic helix mimetics have emerged as powerful molecular scaffolds for the discovery of protein-protein interaction inhibitors. Protein-protein interactions often involve large contact areas, which are often difficult for small molecules to target with high specificity. The hypothesis behind the design of stabilized helices and helix mimetics is that these medium-sized molecules may pursue their targets with higher specificity because of a larger number of contacts. This protocol describes an optimized synthetic strategy for the preparation of stabilized α-helices that feature a carbon-carbon linkage in place of the characteristic N-terminal main-chain hydrogen bond of canonical helices. Formation of the carbon-carbon bond is enabled by a microwave-assisted ring-closing metathesis reaction between two terminal olefins on the peptide chain. The outlined strategy allows the synthesis and purification of a hydrogen bond surrogate (HBS) α-helix in ∼ 1 week.

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Sigma-Aldrich
DIC, 99%
Sigma-Aldrich
Sodium diethyldithiocarbamate trihydrate, ACS reagent
Supelco
Sodium diethyldithiocarbamate trihydrate, ACS reagent, for the determination and separation of heavy metals, ≥99.0%
Sigma-Aldrich
Diisopropylcarbodiimide solution, 1 M in dichloromethane
Sigma-Aldrich
Diisopropylcarbodiimide solution, 1 M in THF