Microglia derived from the axotomized adult rat facial nucleus uptake glutamate and metabolize it to glutamine in vitro.

Neurochemistry international (2016-11-07)
Kazuyuki Nakajima, Tomoyuki Kanamatsu, Maasa Koshimoto, Shinichi Kohsaka
RESUMEN

Microglia in the axotomized adult rat facial nucleus (axoFN) have been shown to highly express a glutamate transporter (GLT-1). The microglia appear to serve as glutamate (Glu) scavengers in the axoFN. However, there is no evidence that the microglia actually have the ability to uptake Glu and convert it to Gln. In this study, we investigated whether axoFN-derived microglia (axoFN-microglia) can uptake Glu and metabolize it to Gln. Microglia obtained by explant culture of axoFN on poly(N-isopropylacrylamide)-grafted dishes were non-invasively sub-cultured onto dishes or wells. Immunoblotting and Glu-uptake experiments revealed that the axoFN-microglia uptake

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Sigma-Aldrich
Pyridinium dichromate, 98%
Sigma-Aldrich
Dihydrokainic acid, ≥98% (HPLC), powder