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Reusable, polyethylene glycol-structured microfluidic channel for particle immunoassays.

Journal of biological engineering (2009-04-30)
Jin-Hee Han, Jeong-Yeol Yoon
ABSTRACT

A microfluidic channel made entirely out of polyethylene glycol (PEG), not PEG coating to silicon or polydimethylsiloxane (PDMS) surface, was fabricated and tested for its reusability in particle immunoassays and passive protein fouling, at relatively high target concentrations (1 mg ml(-1)). The PEG devices were reusable up to ten times while the oxygen-plasma-treated polydimethyl siloxane (PDMS) device could be reused up to four times and plain PDMS were not reusable. Liquid was delivered spontaneously via capillary action and complicated bonding procedure was not necessary. The contact angle analysis revealed that the water contact angle on microchannel surface should be lower than approximately 60 degrees , which are comparable to those on dried protein films, to be reusable for particle immunoassays and passive protein fouling.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Mouse IgG (whole molecule) antibody produced in goat, affinity isolated antibody, lyophilized powder
Sigma-Aldrich
IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder