[Saltar al contenido](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/ES/es) Productos Carrito0 ESES Productos [Inicio de sesión / Registrarse](https://www.sigmaaldrich.com/oidc-sign-in) [Búsqueda de pedido](https://www.sigmaaldrich.com/ES/es/order-lookup) [Pedido rápido](https://www.sigmaaldrich.com/ES/es/quick-order) Carrito0 [Home](https://www.sigmaaldrich.com/ES/en)[Solid Phase Microextraction (SPME)](https://www.sigmaaldrich.com/ES/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction)SPME Direct Immersion Sampling # SPME Direct Immersion Sampling [Learn More About SPME](https://www.sigmaaldrich.com/ES/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction) Direct immersion sampling with solid-phase microextraction (SPME) simplifies extraction of polar analytes that are extremely soluble in many sample matrices. Begin the extraction process by placing the sample into the vial puck on the hotplate. Insert the holder into the sampling stand; passing the needle through the septum that seals the sample vial. Position the fiber below the surface of the liquid and start the timer. The SPME immersion sampling concentrates the dissolved organics on the fiber coating through agitation and heating of the sample. After completion of the extraction with the SPME fiber, retract the fiber into the protective needle and remove it from the sample vial. Inject the SPME needle into the heated GC injection port. Push the plunger down, exposing the fiber to the heat of the injection port and thermally desorbing the collected analyte into the GC column for analysis. __Related Articles__ - [Biodiesel](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/biodiesel) - [Automated Solid Phase Microextraction (SPME) Sampling](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/automated-spme-sampling) - [Bioanalysis with SPME](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/bioanalysis-with-spme) - [BioSPME for Plasma Protein Binding Assay: Details of Method Development and Optimization](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/biospme-plasma-protein-binding-assay) - [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) - [GC/MS/MS Analysis of BPA in Energy Drink](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/gc-ms-ms-analysis-of-bisphenol-a-bpa-in-a-fruit-flavored-energy-drink-cla010218a) - [Rugged SPME Sampling](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) - [SPME Fiber Performance Tips for Volatiles & Semi-Volatiles](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/performance-tips) - [View More](https://www.sigmaaldrich.com/ES/en/search/facet-search?focus=sitecontent&term=facet-search) Arriba __Inicie sesión para continuar.__ Para seguir leyendo, inicie sesión o cree una cuenta. Iniciar sesión__¿No tiene una cuenta?__Registrar An unknown error has occured.