[Saltar al contenido](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/ES/es) Productos Carrito0 ESES Productos ProductosAplicacionesServiciosRecursosApoyo [Inicio de sesión / Registrarse](https://www.sigmaaldrich.com/oidc-sign-in) [Búsqueda de pedido](https://www.sigmaaldrich.com/ES/es/order-lookup) [Pedido rápido](https://www.sigmaaldrich.com/ES/es/quick-order) Carrito0 [Home](https://www.sigmaaldrich.com/ES/en)[Epigenetics](https://www.sigmaaldrich.com/ES/en/applications/genomics/epigenetics)Global DNA Methylation Quantification # Global DNA Methylation Quantification __Savita Bagga__ The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. ## Global DNA Modification Kit The Global DNA Modification Kit ([MDQ1](https://www.sigmaaldrich.com/ES/en/product/SIGMA/MDQ1)), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay. This is a format more friendly to biological researchers and is the first of its kind on the market. __Features and Benefits__ - ELISA-Based Format         – Procedure completes in four easy-to-follow steps         – All reagents supplied, including methylated DNA control - Quickly Measures Changes in DNA Methylation         – Detection limit is 5 ng of fully methylated DNA         – Input DNA may be as low as 10–200 ng - Complete, Flexible, and Fast         – Procedure complete in 3.5 hours         – 96-well format allows option of studying single samples or high-throughput studies - Extended Range of DNA Sample Sources         – May be used with DNA from cells, tissues, plasma, and other body fluids ![MDQ1 Step-wise Protocol](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/epigenetics/mdq1-step-wise-protocol.jpg "MDQ1 Step-wise Protocol") __Figure 1.__Step-wise protocol for using [MDQ1](https://www.sigmaaldrich.com/product/SIGMA/MDQ1). ![Comparison of two differently methylated lots of DNA with MDQ1](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/epigenetics/methylated-lots-of-dna.jpg "Comparison of two differently methylated lots of DNA with MDQ1") __Figure 2.Comparison of two differently methylated lots of DNA with [MDQ1](https://www.sigmaaldrich.com/product/SIGMA/MDQ1)__. Methylation status of two lots of Sss I treated DNA was measured by LC-MS. Varying amounts of DNA was also analyzed via the [MDQ1](https://www.sigmaaldrich.com/product/SIGMA/MDQ1) kit. As demonstrated by the graph, the difference in methylation states can easily be detected and the signal generated is proportional to the amount of DNA used in the procedure. ![Effect of Azacytadine on CHO Cell Global Methylation](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/epigenetics/effect-of-azacytadine.jpg "Effect of Azacytadine on CHO Cell Global Methylation") __Figure 3.Effect of Azacytadine on CHO Cell Global Methylation.__ Azacytadine, a known demethylating agent, was added directly to culture medium. Cells were harvested after 48 hours. DNA was isolated using GenElute™ and global methylation was measured. 40 ng of DNA from treated and non-treated CHO cells was analyzed. ## Materials Lo sentimos, se ha producido un error inesperado Response not successful: Received status code 500 __Related Articles__ - [Carcinogenesis and Epigenetics](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/genomics/epigenetics/carcinogenesis-epigenetics) - [Chromatin Immunoprecipitation (ChIP) Troubleshooting](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/genomics/epigenetics/chp1-faqs) - [DNA Methylation & Bisulfite Conversion](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/genomics/epigenetics/dna-methylation-bisulfite-conversion) - [Epigenetics Overview](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/genomics/epigenetics/epigenetics-overview) - [Introduction to DNA Methylation](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/genomics/epigenetics/introduction-to-dna-methylation) - [p53: DNA Damage Response and Cancer](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/genomics/epigenetics/p-fiftythree-dna-damage-response-and-cancer) - [Guides to Cell Number for ChIP and Endpoint Analysis](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/protein-biology/protein-and-nucleic-acid-interactions/cell-number-for-chip-and-endpoint-analysis) - [Chromatin Immunoprecipitation (ChIP)](https://www.sigmaaldrich.com/ES/en/technical-documents/technical-article/protein-biology/protein-and-nucleic-acid-interactions/chip-immunoprecipitation-washing-and-elution) - [View More](https://www.sigmaaldrich.com/ES/en/search/facet-search?focus=sitecontent&term=facet-search) __Related Product Categories__ - [Epigenetics](https://www.sigmaaldrich.com/ES/en/products/molecular-biology-and-functional-genomics/epigenetics) - [Environmental Accounting Supplier Toolkit](https://www.sigmaaldrich.com/ES/en/life-science/ssbi/sustainability-toolkits/environmental-accounting-supplier-toolkit) - [Renewable Electricity Toolkit](https://www.sigmaaldrich.com/ES/en/life-science/ssbi/sustainability-toolkits/renewable-electricity-toolkit) - [Fluorescent Lipid Probes - Avanti Research™](https://www.sigmaaldrich.com/ES/en/technical-documents/product-supporting/biochemicals/fluorescent-lipid-probes-avanti-research) - [Crown Ethers and Cryptands for Advanced Applications](https://www.sigmaaldrich.com/ES/en/technical-documents/product-supporting/crown-ethers-cryptands-advanced-applications) - [Purification of Extracellular Vesicles with Eshmuno® Q Resin and Natrix® Q Membrane](https://www.sigmaaldrich.com/ES/en/technical-documents/product-supporting/membrane-chromatography/purification-extracellular-vesicles) - [Membrane Prefilters Improve Sterilizing Filter Capacity and Process Economics in Bioprocessing](https://www.sigmaaldrich.com/ES/en/technical-documents/product-supporting/prefilters-bioburden-control/membrane-prefiltration-improves-sterilizing-filter-capacity-and-process-economics) - [Next Generation Virus Filtration Solutions](https://www.sigmaaldrich.com/ES/en/technical-documents/product-supporting/virus-filters/next-generation-virus-filtration-solutions) - [View More](https://www.sigmaaldrich.com/ES/en/search/facet-search?focus=sitecontent&term=facet-search) Arriba __Inicie sesión para continuar.__ Para seguir leyendo, inicie sesión o cree una cuenta. 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