324682

Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

• Synonym(s): Elastase, Porcine Pancreas, High Purity, Crystallized
• CAS Number: 39445-21-1
• EC Number: 3.4.21.36 (BRENDA, IUBMB)
• MDL number: MFCD00130998
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• Quality Level: 100
• description: Merck USA index - 14, 3535
• form: lyophilized
• specific activity: ≥25 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• solubility: distilled water: 10 mg/mL
• foreign activity: Chymotrypsin ≤0.2%
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α₂-macroglobulin.

Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25°C, pH 8.0.

Physical form

Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.

Reconstitution

Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.

Other Notes

Largman, C. et al. 1976. Biochemestry15, 2491.
Mandl, I. 1962. Methods in Enzymol.5, 665.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3