324713

Glu-C Protease

Selectively cleaves at glutamic acid residues, suitable for mass spectromety, from Staphylococcus aureus

• Synonym(s): Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus, V8 Protease
• CAS Number: 66676-43-5
• EC Number: 3.4.21.19 (BRENDA, IUBMB)
• MDL number: MFCD01324998
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• Product Name: Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus,
• biological source: Staphylococcus aureus
• Quality Level: 100
• grade: Proteomics Grade
• form: lyophilized
• specific activity: ≥10 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α₂-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA69, 3506.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable