D3812

DirectLoad^™ 50 bp DNA Step Ladder

Name: DirectLoad<SUP>™</SUP> 50 bp DNA Step Ladder
Brand: SIGMA
Price: 182 EUR

ready-to-use marker for DNA electrophoresis

Synonym(s):

DirectLoad^™ 50 bp DNA Marker, Ready-to-use 50 bp DNA Marker, Ready-to-use DirectLoad^™ 50 bp DNA Marker, Ready-to-use DirectLoad^™ 50 bp DNA Step Ladder

Slide 1 of 1

Select a Size

1 VIAL

€182.00

Please contact Customer Service for Availability

About This Item

NACRES:

NA.25

UNSPSC Code:

41105335

Key Documents

View All Documentation

Product Name

DirectLoad^™ 50 bp DNA Step Ladder, ready-to-use marker for DNA electrophoresis

form

(liquid)

usage

sufficient for 100 loads

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Quality Level

200

Related Categories

Nucleic Acid Electrophoresis & Hybridization

Nucleic Acid Gel Electrophoresis Equipment and Systems

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	S7025	D3687	D3937
Sigma-Aldrich D3812 DirectLoad^™ 50 bp DNA Step Ladder	Sigma-Aldrich S7025 50 bp DNA Step Ladder	Sigma-Aldrich D3687 DirectLoad^™ PCR 100 bp Low Ladder	Sigma-Aldrich D3937 DirectLoad^™ 1 kb DNA Ladder
form (liquid)	form liquid	form liquid	form liquid
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 200
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
usage sufficient for 100 loads	usage 100 uses	usage 75 uses	usage 100 uses
suitability suitable for electrophoresis (DNA)	suitability suitable for electrophoresis (DNA)	suitability suitable for electrophoresis (DNA)	suitability suitable for electrophoresis (DNA)
NA electrophoresis	NA electrophoresis, ladder, marker	-	NA electrophoresis, ladder, marker

Application

DirectLoad^™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.[1]

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

Ready-to-load
Easy-to-use
Popular band sizes

General description

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sigma′s DirectLoad^™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.

Description

Pricing

D0428

1 kb DNA Ladder, for DNA electrophoresis

D7058

DirectLoad^™ Wide Range DNA Marker, ready-to-use marker for DNA electrophoresis

D3937

DirectLoad^™ 1 kb DNA Ladder, ready-to-use marker for DNA electrophoresis

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Solid-phase reverse transfection for intracellular delivery of functionally active proteins

Bulkescher R, et al.

Genome Research, 27(10), 1752-1758 (2017)

Detection of pathogenic Vibrio parahaemolyticus through biochemical and molecular-based methodologies in coastal waters of the Gulf of Trieste (North Adriatic Sea).

Cinzia Fabbro et al.

FEMS microbiology letters, 307(2), 158-164 (2010-04-24)

Culturable vibrios were isolated from seawater collected during an annual sampling study performed along the Gulf of Trieste coast (Northern Adriatic Sea), and conventional culturing and identification methods were used to investigate the presence of Vibrio parahaemolyticus. Biochemically selected Vibrio

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Protocols

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

DirectLoad™ 50 bp DNA Step Ladder