NDEGLY

Native Protein Deglycosylation Kit

Name: Native Protein Deglycosylation Kit
Brand: SIGMA
Price: 858 EUR

Synonym(s):

Protein Deglycosylation Kit

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1 KIT

€858.00

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Key Documents

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conjugate

(N-linked)

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Related Categories

Glycobiology Profiling Tools

Proteins & Enzymes

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This Item	324727	324725	324726
Sigma-Aldrich NDEGLY Native Protein Deglycosylation Kit	Sigma-Aldrich 324727 Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli	Sigma-Aldrich 324725 Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli	Sigma-Aldrich 324726 Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
conjugate (N-linked)	conjugate (N-linked)	conjugate (N-linked)	conjugate (N-linked)

Application

Native Protein Deglycosylation Kit has been for N-deglycosylation of various enzymes such as laccase,[1] tomato nuclease TBN1 and endo-β-1,3-glucanase.[2]

General description

All complex oligosaccharides can be reduced to the trimannosylchitobiose core by treatment of the glycoproteins with neuraminidase, β-galactosidase, and N-acetylglucosaminidase. Fucosidases may be required in some situations. The remaining trimannosylchitobiose core structures can be removed with endoglycosidase F3. Biantennary and triantennary structures can be immediately removed by endoglycosidases F2 and F3, respectively. Oligomannose and hybrid structures can be removed by Endoglycosidase F1.
For more information on each type of endoglycosidase, please refer to the Bulletin.

The Native Protein Deglycosylation is designed for the deglycoslylation of N-linked oligosaccharides from PNGase F-resistant native proteins. Endoglycosidases F1, F2, and F3 are less sensitive to protein conformation than PNGase F and are more suitable for removal of all classes of N-linked oligosaccharides without protein denaturation.

Preparation Note

The NDEGLY Kit ships on wet ice and storage at 2–8 °C is recommended. This kit may be used for at least 1 year when stored as indicated. Several days exposure to ambient temperatures will not reduce the activity of the enzymes in this kit.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Prolactin released in vitro from the pituitary of lactating, pregnant, and steroid-treated female or male rats stimulates prolactin secretion from pituitary lactotropes of male rats.

Flavio Mena et al.

Neuroendocrinology, 91(1), 77-93 (2009-07-11)

We have previously shown that soluble factor(s) in conditioned media (CM) from the central and peripheral regions of the anterior pituitary (AP) gland of lactating rats promoted the in vitro dose-related release of prolactin (PRL) from pituitary glands of male

N-glycosylation and homodimeric folding significantly enhance the immunoreactivity of Mycobacterium tuberculosis virulence factor CFP32 when produced in the yeast Pichia pastoris.

Chaouki Benabdessalem et al.

Biochemical and biophysical research communications, 516(3), 845-850 (2019-07-03)

We previously reported that immunoreactivity of recombinant CFP32 (Rv0577), a virulence factor of Mycobacterium tuberculosis, was higher when produced in transformed Pichia pastoris as compared to transformed E. coli. In this study, we show that this difference is partly due to

Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization

Kolomytseva MP, et al.

International Journal of Biological Macromolecules (2019)

Emergence of functional delta-opioid receptors induced by long-term treatment with morphine.

Junyi Ma et al.

Molecular pharmacology, 69(4), 1137-1145 (2006-01-10)

Opioid analgesics remain the choice for the treatment of moderate to severe pain. Recent research has established that the mu-opioid receptor is predominantly responsible for mediating many opioid actions, including analgesia and opioid tolerance. However, the function of delta-opioid receptors

N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity

Podzimek T, et al.

Plant Science (2018)

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