SRP5096

ULK1 (1-649), active, GST tagged human

Name: ULK1 (1-649), active, GST tagged human
Brand: SIGMA
Price: 570 EUR

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

ATG1, FLJ38455, UNC51, Unc51.1

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SRP5096-10UG

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

Specific activity:

46-62 nmol/min·mg

Assay:

≥70% (SDS-PAGE)

Recombinant:

expressed in baculovirus infected Sf9 cells

Key Documents

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recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

46-62 nmol/min·mg

mol wt

~125 kDa

NCBI accession no.

BC111603

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ULK1(8408)

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Show Differences

1 of 4

This Item	SRP0252	SRP5364	SRP5089
Sigma-Aldrich SRP5096 ULK1 (1-649), active, GST tagged human	Sigma-Aldrich SRP0252 ULK Active human	Sigma-Aldrich SRP5364 ULK4, GST tagged human	Sigma-Aldrich SRP5089 TBK1, active, GST tagged human
Gene Information human ... ULK1(8408)	Gene Information human ... ULK1(8408)	Gene Information human ... ULK4(54986)	Gene Information human ... TBK1(29110)
specific activity 46-62 nmol/min·mg	specific activity -	specific activity -	specific activity 259-351 nmol/min·mg
assay ≥70% (SDS-PAGE)	assay ≥40% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
form buffered aqueous glycerol solution	form aqueous solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in FreeStyle^™ 293-F cells	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
mol wt ~125 kDa	mol wt 113 kDa	mol wt ~97 kDa	mol wt ~105 kDa

General description

Unc-51 like autophagy activating kinase 1 (ULK1) is a serine/threonine protein kinase which forms a component of the ULK1 protein kinase complex. This protein complex also contains the adaptor proteins ATG (autophagy-related) 13, RB1CC1/FIP200 (RB1-inducible coiled-coil 1), and ATG101. The gene encoding this protein is localized on human chromosome 12q24.33.[1][2]

Application

GST-ULK1 has been used in in vitro kinase assay.[3]

Biochem/physiol Actions

Unc-51 like autophagy activating kinase 1 (ULK1) protein kinase plays a crucial role in the control of autophagy initiation. This protein is responsible for activating a cascade of signaling pathways which results in the formation of phagosomes. ULK1 is also required for the control of the expression of key interferon-stimulated genes (ISGs).[1][4][5]

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

W068-1

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Computational analysis of an autophagy/translation switch based on mutual inhibition of MTORC1 and ULK1.

Szymanska Pp

PLoS ONE (2015)

Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy.

Driebren S

Autophagy (2015)

Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy

Stefan D

Autophagy (2015)

Missense Mutation in Pseudouridine Synthase 1 (PUS1) Causes Mitochondrial Myopathy and Sideroblastic Anemia (MLASA)

YelenaBykhovskaya

The American Journal on Addictions (2004)

Central role of ULK1 in type I interferon signaling.

Saleiro D

Cell Reports (2015)

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