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Phosphorothioate Oligonucleotides
Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns.
TRC Design and pLKO.1 Vector FAQs
MISSION® shRNA clones in pLKO.1-Puro vector allow bacterial culture propagation and mammalian cell selection for gene silencing studies.
Efficient Gene Silencing with MISSION® TRC shRNA
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
shRNA Process and Diagram
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
siRNA Design, Manufacturing, Transfection & Detection
RNA interference (RNAi) is a natural biological mechanism wherein short inhibitory RNA (siRNA) duplexes induce potent inhibition of gene expression.
Water for Nucleic Acid Gel Electrophoresis
Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.
Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher
Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.
Array CGH Analysis with Whole Genome Amplification
aCGH technology evolves for high-res chromosomal analysis, needing a large DNA input per sample, advancing genetic research.
Multiplex PCR Assay for Damaged DNA
Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
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