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MDQ1

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Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

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Synonym(s):
DNA Methylation Quantification, DNA methylation shift
NACRES:
NA.54

Quality Level

200

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MOD50S7806S7855
Imprint® Methylated DNA Quantification Kit To measure global DNA methylation shifts from as low as 10 ng DNA

Sigma-Aldrich

MDQ1

Imprint® Methylated DNA Quantification Kit

Imprint® DNA Modification Kit For bisulfite DNA conversion & purification

Sigma-Aldrich

MOD50

Imprint® DNA Modification Kit

CpG WIZ™ Prader-Willi/Angelman -Methylation specific PCR assay The CpG WIZ Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation-specific PCR (MSP).

Sigma-Aldrich

S7806

CpG WIZ™ Prader-Willi/Angelman -Methylation specific PCR assay

CpGenome Rat Methylated Genomic DNA Standard Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.

Sigma-Aldrich

S7855

CpGenome Rat Methylated Genomic DNA Standard

General description

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Application

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Features and Benefits

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • M8570Methylated Control DNA, 50 ng/μLSDS

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Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit, 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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Methylated Control DNA, 50 ng/μL

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290 - H317 - H412

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

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Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Mounira Krifa et al.
Journal of experimental & clinical cancer research : CR, 32, 30-30 (2013-05-22)
Several reports have described the potential effects of natural compounds as anti-cancer agents in vitro as well as in vivo. The aim of this study was to evaluate the anti-cancer effect of Limoniastrum guyonianum aqueous gall extract (G extract) and
Rauf Ahmad Najar et al.
The Journal of nutritional biochemistry, 53, 121-132 (2017-12-09)
The present study has been designed to determine the effect of folate modulation (deficiency/supplementation) with aging on the promoter methylation of tumor suppressor and proto-oncogenes to understand the underlying mechanism of epigenetic alterations. Folate deficiency was induced for 3 and
Svetlana I Novikova et al.
PloS one, 3(4), e1919-e1919 (2008-04-03)
Previous studies documented significant behavioral changes in the offspring of cocaine-exposed mothers. We now explore the hypothesis that maternal cocaine exposure could alter the fetal epigenetic machinery sufficiently to cause lasting neurochemical and functional changes in the offspring. Pregnant CD1
Maiko Suzuki et al.
Molecular medicine reports, 1(4), 465-471 (2008-07-01)
Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis in oral squamous cell carcinoma (OSCC). In this study, we used the novel DNA methyltransferase inhibitor zebularine (Zeb) to investigate epigenetic influences on the secretion of VEGF-A in the
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Articles

Global DNA Methylation Quantification

The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. The Global DNA Modification Kit (MDQ1), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay.

Protocols

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol

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