1.08218

Trifluoroacetic acid

(25% solution in water) for protein sequenation

• Synonym(s): Trifluoroacetic acid solution, TFA solution, TFA
• Linear Formula: CF₃COOH
• CAS Number: 76-05-1
• Molecular Weight: 114.02
• Beilstein: 5446934
• MDL number: MFCD00004169

Properties

• Quality Level: 200
• form: liquid
• composition: Water, 74.5-75.5% Karl Fischer
• concentration: 24.5-25.5% (trifluoroacetic acid)
• density: 1.12 g/cm³ at 20 °C
• storage temp.: 15-25°C
• SMILES string: OC(=O)C(F)(F)F
• InChI: 1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: DTQVDTLACAAQTR-UHFFFAOYSA-N

Description

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Packaging

50, 50 ml

Analysis Note

Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314 - H412
• Precautionary Statements: P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable