Merck
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1.09204

Sigma-Aldrich

Giemsa′s azur eosin methylene blue solution

for microscopy

Quality Level

form

liquid

autoignition temp.

455 °C

expl. lim.

5.5-44 % (v/v) Methanol)

IVD

for in vitro diagnostic use

pH

6.1-7.0 (20 °C in H2O, undiluted)

bp

>65 °C/1013 hPa

transition temp

flash point 18 °C

density

0.99 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

Giemsa′s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa′s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald′s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa′s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Packaging

25 l in Barrel PE/met
100 ml in Glass bottle
1, 2.5 l in Plastic bottle
500 ml in Plastic bottle

Application

For microscopy. For the staining of blood and bone-marrow smears, paraffin sections and clinical-cytological specimens

Analysis Note

For staining results, please refer to the Instructions for Use link under the Documents Section below.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

64.4 °F

Flash Point(C)

18 °C


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificate of Analysis

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Certificate of Quality

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