Merck
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1.09253

Sigma-Aldrich

Papanicolaou′s solution 1a Harris′ hematoxylin solution

for cytology

Synonym(s):
Hematoxylin solution acc. to Harris, Colour solution acc. to Papanicolaou, Harris′ Hematoxylin solution

Quality Level

form

liquid

IVD

for in vitro diagnostic use

pH

2.5-3.0 (20 °C in H2O)

bp

100 °C/1013 hPa

density

1.04 g/cm3 at 20 °C

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

Papanicolaou′s solution 1a Harris hematoxylin solution - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou′s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Product number 1.05175), Papanicolaou′s solution 1a Harris hematoxylin solution, (Product number 1.09253) or Papanicolaou′s solution 1b Hematoxylin solution S, (Product number 1.09254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by (Papanicolaou′s solution 2b Orange II solution, (Product number 1.106887) or Papanicolaou′s solution 2a Orange G solution (OG6), (Product number 1.06888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou′s solution 3a polychromatic solution EA 31, (Product number 1.09271), Papanicolaou′s solution 3b polychromatic solution EA 50, (Product number 1.09272), Papanicolaou′s solution 3c polychromatic solution EA 65, (Product number 1.09270), or Papanicolaou′s solution 3d polychromatic solution EA 65, (Product number 1.09269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou′s solution 1a Harris hematoxylin solution (Product number 1.09253) and Papanicolaou′s solution 1b Hematoxylin solution S (Product number 1.09254) give a blue to dark violet staining result with clinical specimen material.
A 500 ml bottle of either solution is sufficient for 1500 -2500 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Packaging

1, 2.5 l in Plastic bottle
500 ml in Plastic bottle

Analysis Note

Suitability for microscopy (Vaginal smear): passes test
Nuclei: blue to dark violet
Cyanophilic cytoplasm: blue-green
Eosinophilic cytoplasm: pink

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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