Merck
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8.18710

Sigma-Aldrich

Urea

for synthesis

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Synonym(s):
Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
MDL number:
EC Index Number:
200-315-5

vapor pressure

<0.1 hPa ( 20 °C)

Quality Level

potency

8471 mg/kg LD50, oral (Rat)
8200 mg/kg LD50, skin (Rat)

pH

9 (20 °C, 100 g/L in H2O)

mp

132-135 °C (lit.)

solubility

1000 g/L

density

1.335 g/mL at 25 °C (lit.)

bulk density

720‑760 kg/m3

storage temp.

2-30°C

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

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Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Analysis Note

Assay (ex N): ≥ 99.0 %
Melting range (lower value): ≥ 132 °C
Melting range (upper value): ≤ 135 °C
Identity (IR): passes test

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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