Merck
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DNTPM-RO

Roche

PCR Nucleotide Mix

solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

All Photos(2)
Synonym(s):
PCR | Nucleoide

form

solution

Quality Level

100

usage

sufficient for 2,500 reactions (04638956001)
 sufficient for 5,000 reactions (11814362001)
 sufficient for 500 reactions (11581295001)

packaging

pkg of 10 × 200 μL (11814362001 [10mM, each])
pkg of 200 μL (11581295001 [10 mM, each])
pkg of 5 × 200 μL (04638956001 [10 mM, each])

manufacturer/tradename

Roche

storage temp.

−20°C

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This Item
11277081001D729511636103001
PCR Nucleotide Mix solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

Roche

DNTPM-RO

PCR Nucleotide Mix

Hexanucleotide Mix solution, pkg of 100 μL, sufficient for 50 labeling reactions

Roche

11277081001

Hexanucleotide Mix

Deoxynucleotide Mix, 10 mM Molecular Biology Reagent

Sigma-Aldrich

D7295

Deoxynucleotide Mix, 10 mM

PCR Master (50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase), sufficient for 200 reactions, kit of 1 (2 components), suitable for PCR

Roche

11636103001

PCR Master

form

solution

form

solution

form

liquid

form

-

packaging

pkg of 10 × 200 μL (11814362001 [10mM, each]), pkg of 200 μL (11581295001 [10 mM, each]), pkg of 5 × 200 μL (04638956001 [10 mM, each])

packaging

pkg of 100 μL

packaging

-

packaging

kit of 1 (2 components)

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

100

usage

sufficient for 2,500 reactions (04638956001)

usage

sufficient for 50 labeling reactions

usage

-

usage

sufficient for 200 reactions, (50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase)

General description

PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.

Application

  • The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR
  • RT-PCR
  • cDNA synthesis
  • Primer extension

Features and Benefits

The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

Improve yield and performance
  • Choose a convenient ready-to-use mix of all 4 nucleotides.
  • Achieve the highest PCR and RT-PCR sensitivity.
  • Insist on highest purity (>99%) nucleotides.

Packaging

1 set containing 4 ready-to-use mixes

Quality

Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.

Preparation Note

Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description
  • dATP, PCR Grade, sodium salt 10 mM
  • dCTP, PCR Grade, sodium salt 10 mM
  • dGTP, PCR Grade, sodium salt 10 mM
  • dTTP, PCR Grade, sodium salt 10 mM

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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