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33247

Sigma-Aldrich

Urea

puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

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Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

grade

ACS reagent
puriss. p.a.

Quality Level

Agency

reag. Ph. Eur.

Assay

≥99%
99-101% (calc. on dry substance)

form

solid

impurities

alkalic reac. substances, complies
≤0.0005% heavy metals (as Pb)
≤0.05% ammonium (NH4)
≤0.1% biuret

ign. residue

≤0.01% (as SO4)

loss

≤1.0% loss on drying, 105°C, 1 h

mp

132-135 °C (lit.)
132-135 °C

solubility

H2O: soluble 480 g/L at 20 °C

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Fe: ≤2 mg/kg

suitability

in accordance for appearance of solution
in accordance for identity

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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This Item
15604U488451456
Urea puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

Sigma-Aldrich

33247

Urea

-
Urea puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

Sigma-Aldrich

15604

Urea

-
Urea meets USP testing specifications

Sigma-Aldrich

U4884

Urea

-
Urea BioUltra, for molecular biology, 99% (T)

Sigma-Aldrich

51456

Urea

Premium Grade
form

solid

form

solid

form

solid

form

powder or crystals

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

solubility

H2O: soluble 480 g/L at 20 °C

solubility

H2O: soluble 480 g/L at 20 °C

solubility

H2O: soluble 480 g/L at 20 °C

solubility

H2O: 8 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

density

1.335 g/mL at 25 °C (lit.)

density

1.335 g/mL at 25 °C (lit.)

density

1.335 g/mL at 25 °C (lit.)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.
Urea is the most commonly used slow release nitrogen fertilizer.

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Urea has been used in the following studies:
  • Synthesis of SnO2 nanoparticles.
  • Synthesis of urethanes, ureides and semicarbazides.
  • Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.
  • Protein electrophoretic studies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The Fertilizer Encyclopedia, 709-709 (2009)
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