Merck
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80457

Supelco

Trifluoroacetic acid

eluent additive for LC-MS, LiChropur, ≥99.0% (GC)

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Synonym(s):
TFA
Linear Formula:
CF3COOH
CAS Number:
Molecular Weight:
114.02
Beilstein:
742035
EC Number:
MDL number:

grade

eluent additive for LC-MS

Quality Level

vapor density

3.9 (vs air)

vapor pressure

97.5 mmHg ( 20 °C)

Assay

≥99.0% (GC)

form

liquid

technique(s)

LC/MS: suitable

impurities

≤0.05% water
≤50 mg/kg total sulfur (as SO4)

refractive index

n20/D 1.3 (lit.)

bp

72.4 °C (lit.)

mp

−15.4 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfite (SO32-): ≤10 mg/kg

cation traces

Ag: ≤0.05 mg/kg
Al: ≤0.1 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.5 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.1 mg/kg
Co: ≤0.05 mg/kg
Cr: ≤0.1 mg/kg
Cu: ≤0.05 mg/kg
Fe: ≤1.0 mg/kg
K: ≤1.0 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤1.0 mg/kg
Mn: ≤0.1 mg/kg
Mo: ≤0.05 mg/kg
NH4+: ≤10 mg/kg
Na: ≤1.0 mg/kg
Ni: ≤0.1 mg/kg
Pb: ≤0.05 mg/kg
Sr: ≤0.05 mg/kg
Tl: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

UV absorption

λ: 260 nm Amax: ≤0.9
λ: 270 nm Amax: ≤0.1
λ: 280 nm Amax: ≤0.08
λ: 290 nm Amax: ≤0.07
λ: 300 nm Amax: ≤0.06
λ: 320 nm Amax: ≤0.05

SMILES string

OC(C(F)(F)F)=O

suitability

passes test for LC-MS

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

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This Item
T6508302031299537
Trifluoroacetic acid eluent additive for LC-MS, LiChropur™, ≥99.0% (GC)

Supelco

80457

Trifluoroacetic acid

Trifluoroacetic acid ReagentPlus®, 99%

Sigma-Aldrich

T6508

Trifluoroacetic acid

Trifluoroacetic acid suitable for HPLC, ≥99.0%

Sigma-Aldrich

302031

Trifluoroacetic acid

Trifluoroacetic acid ≥99%, for protein sequencing

Sigma-Aldrich

299537

Trifluoroacetic acid

vapor density

3.9 (vs air)

vapor density

3.9 (vs air)

vapor density

3.9 (vs air)

vapor density

3.9 (vs air)

vapor pressure

97.5 mmHg ( 20 °C)

vapor pressure

97.5 mmHg ( 20 °C)

vapor pressure

97.5 mmHg ( 20 °C)

vapor pressure

97.5 mmHg ( 20 °C)

assay

≥99.0% (GC)

assay

99%

assay

≥99.0%

assay

≥99%

form

liquid

form

liquid

form

liquid

form

liquid

impurities

≤0.05% water, ≤50 mg/kg total sulfur (as SO4)

impurities

≤0.05% water

impurities

<0.050% water

impurities

<0.05% water

General description

Trifluoroacetic acid is commonly used as a mobile phase additive in liquid chromatography-mass spectrometry (LC-MS).
Features:
  • Extensive QC testing ensures the highest specifications.        
  • Optimized to improve ionization and resolution.        
  • Extremely low levels of inorganic and organic impurities.       
  • Manufactured specifically for accurate and fast LC-MS.

Application

Trifluoroacetic acid may be used as a mobile phase additive for the analysis of basic compounds in biological samples using hydrophilic interaction liquid chromatography-electrospray ionization coupled to tandem mass spectrometry (HILIC–ESI/MS/MS) and reversed-phase liquid chromatography coupled to tandem mass spectrometry (RP-LC-MS/MS).

Packaging

Clear borosilicate glass, high hydrolytic resistance (Type I)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

212.0 °F - Pensky-Martens closed cup

Flash Point(C)

> 100 °C - Pensky-Martens closed cup


Certificates of Analysis (COA)

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705578-5MG-PW

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1000309185

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Simple means to alleviate sensitivity loss by trifluoroacetic acid (TFA) mobile phases in the hydrophilic interaction chromatography?electrospray tandem mass spectrometric (HILIC?ESI/MS/MS) bioanalysis of basic compounds
Shou ZW and Naidong W
Journal of Chromatography. B, Biomedical Sciences and Applications, 825(2), 186-192 (2005)

Articles

Difluoroacetic Acid as an Effective Mobile Phase Modifier for the LC-UV/MS Analysis of Proteins

The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.

Middle-Up Analysis of Antibodies by Reversed-Phase Chromatography

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Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.

Protocols

Determination of Lactose in Dairy Products Using TLC-MS

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Workflow for High-Throughput Glycoprofiling of Rituximab

Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.

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A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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