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B0149

Sigma-Aldrich

Brilliant Blue R

Dye content ~50 %, Technical grade

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Synonym(s):
Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein:
5718025
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder

composition

Dye content, ~50%

solubility

H2O: 10 mg/mL

suitability

suitable for detection of protein bands following electrophoresis.

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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1 of 4

This Item
27816B8647B7920
vibrant-m

B0149

Brilliant Blue R

vibrant-m

27816

Brilliant Blue R

vibrant-m

B8647

Brilliant Blue R Concentrate

vibrant-m

B7920

Brilliant Blue R

solubility

H2O: 10 mg/mL

solubility

-

solubility

-

solubility

H2O: 1 mg/mL

form

powder

form

powder

form

methanol solution

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Dye content, ~50%

composition

-

composition

-

composition

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250.

Application

Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).Its other applications include:
  • as a protein stain on epoxy-embedded tissue sections
  • for quantitative micro densitometry of protein in sections
  • for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes.
For detection of protein bands following electrophoresis.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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