This product is a crude bile extract from ox gall bladder. B3883 is soluble in water at 50 mg per mL resulting in a turbid dark brown to green suspension. The natural concentration is 1 gram of solid material with 11.5 grams of water. We do not have more detailed information about using it in preparing a blank matrix.
B3883
Bile bovine
dried, unfractionated
Synonym(s):
Ox gall powder
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About This Item
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sterility
non-sterile
Quality Level
form
dried, unfractionated
color
yellow-brown
solubility
water: soluble 50 mg/mL
application(s)
microbiology
Related Categories
1 of 4
This Item | B8381 | B8756 | 48305 |
|---|---|---|---|
| form dried, unfractionated | form powder | form powder | form powder |
| application(s) microbiology | application(s) - | application(s) microbiology | application(s) microbiology |
| sterility non-sterile | sterility - | sterility non-sterile | sterility non-sterile |
| solubility water: soluble 50 mg/mL | solubility - | solubility distilled water: freely soluble | solubility H2O: 0.1 g/mL, clear, faintly yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| color yellow-brown | color - | color - | color - |
General description
Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.
Application
Bile bovine has been used:
- in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition[1]
- as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology[2]
- as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583[3]
Biochem/physiol Actions
Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.[4]
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Helena Šeme et al.
Food research international (Ottawa, Ont.), 94, 45-53 (2017-03-16)
Malabsorption of dietary sugars is a common cause of gastrointestinal discomfort, affecting up to one in three people with debilitating symptoms, such as abdominal pain, osmotic diarrhoea, bloating and flatulence. Besides dietary interventions, it has been suggested that ingestion of
Xin Wen et al.
Food chemistry, 257, 36-43 (2018-04-07)
As hydrolysis of carotenoid esters is believed to be highly efficient in vivo, their insufficient hydrolysis in in vitro-digestion models, particularly, regarding zeaxanthin diesters, is a current issue. Therefore, in this study, several factors related to the enzymatic hydrolysis were
Impact of bile salt adaptation of Lactobacillus delbrueckii subsp. lactis 200 on its interaction capacity with the gut
Burns P, et al.
Research in Microbiology, 162(8), 782- 790 (2011)
Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties
Guo XH, et al.
Anaerobe, 16(4), 321- 326 (2010)
Bile acid patterns in commercially available oxgall powders used for the evaluation of the bile tolerance ability of potential probiotics
Hu PL, et al.
PLoS ONE, 13(3), e0192964-e0192964 (2018)
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Hi, I am trying to prepare blank bile matrix using this bovine bile powder. Do you have any recommendation of mixing ratio? Thank you
1 answer-
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WHAT IS THE PROPER TEMPERATURE FOR STORAGE?
1 answer-
Item B3883 can be stored at room temperature.
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Can you tell us the exact bile salt concentration?
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however other components such as glycocholic and taurocholic acid are also present.
Helpful?
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I need to prepare bile salt (10 mM). May I know the content of Bile in this product? Or the molecular weight of this products? Thanks.
1 answer-
This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however, other components such as glycocholic and taurocholic acid are also present.
Helpful?
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