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09978

Sigma-Aldrich

Ammonium sulfate

BioUltra, ≥99.0% (T)

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Synonym(s):
Mascagnite
Linear Formula:
(NH4)2SO4
CAS Number:
Molecular Weight:
132.14
EC Number:
MDL number:
eCl@ss:
38030408
PubChem Substance ID:
NACRES:
NA.26

product line

BioUltra

Quality Level

Assay

≥99.0% (T)

form

crystals

impurities

insoluble matter, passes filter test

ign. residue

≤0.01% (as SO4)

loss

≤0.1% loss on drying, 110 °C

pH

5.0-6.0 (25 °C, 1 M in H2O)

mp

>280 °C (dec.) (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

1.77 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
nitrate (NO3-): ≤50 mg/kg
phosphate (PO43-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.2 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤50 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤2 mg/kg
Sr: ≤5 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.037
λ: 280 nm Amax: ≤0.030

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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This Item
A49151.012171.01211
Ammonium sulfate BioUltra, ≥99.0% (T)

Sigma-Aldrich

09978

Ammonium sulfate

Premium Grade
Ammonium sulfate ACS reagent, ≥99.0%

Sigma-Aldrich

A4915

Ammonium sulfate

-
Ammonium sulfate for analysis EMSURE® ACS,ISO,Reag. Ph Eur

Supelco

1.01217

Ammonium sulfate

-
Ammonium sulfate for biochemistry

Millipore

1.01211

Ammonium sulfate

-
assay

≥99.0% (T)

assay

≥99.0%

assay

≥99.5% (alkalimetric)

assay

-

form

crystals

form

powder or crystals

form

solid

form

solid

impurities

insoluble matter, passes filter test

impurities

≤0.005% Insoluble matter

impurities

≤0.001% Insoluble matter

impurities

-

ign. residue

≤0.01% (as SO4)

ign. residue

≤0.005%

ign. residue

≤0.005% (as sulfate)

ign. residue

-

loss

≤0.1% loss on drying, 110 °C

loss

-

loss

≤0.1% loss on drying, 105 °C

loss

-

General description

Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.

Application

Ammonium sulfate has been used:
  • to prepare mobile phase A for hydrophobic interaction chromatography (HIC)
  • as reference material for the thermal decomposition of NH4NO3 (S1–S6) thermal decomposition to N2O (S1-N2O–S6-N2O)
  • to prepare the stock solution for M63 medium
  • as a reagent in molecular biology and chromatography
  • in the precipitation and fractionation of proteins
  • in the purification of antibodies
  • for the crystallographic analysis of nucleic acids and proteins
  • in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography
  • for Sepharose chromatography of tRNAs using reverse salt gradients
  • in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis
  • HPLC analysis of phospholipids

Biochem/physiol Actions

Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.

Other Notes

The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose chromatography of tRNAs using reverse salt gradients.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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T.D. Pollard
Methods in Enzymology, 85, 334-334 (1982)
Sepharose chromatography of transfer ribonucleic acids using reverse salt gradients.
G W Hatfield
Methods in enzymology, 59, 215-218 (1979-01-01)
Protein crystallization: the growth of large-scale single crystals.
G L Gilliland et al.
Methods in enzymology, 104, 370-381 (1984-01-01)
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Acta crystallographica. Section D, Biological crystallography, 68(Pt 11), 1450-1459 (2012-10-24)
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