Merck
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24365C

SAFC

EX-CELL® CD CHO Fusion

chemically defined cell culture medium

NACRES:
NA.71

Quality Level

description

Animal-component free
without L-glutamine, without hypoxanthine, without sodium bicarbonate, without thymidine

form

dry powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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Application

EX-CELL ™ CD CHO FUSION is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macromolecules allows for isolation and purification of secreted proteins from the cells.

This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up, and to provide an appropriate medium for the culture of CHO cells using the Glutamine Synthetase, or GS System™. This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase (DHFR) gene amplification systems.

Quantity

Formulated to contain 20.1 grams of powder per liter of medium.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Articles

Preparing CHO Cells for Higher Productivity by Optimizing a Perfused Seed Train

This application note describes the benefits and performance of Cellvento® 4CHO-X Expansion Medium designed for multiple cell expansion steps from vial thaw to N-1 stages to generate higher biomass for inoculating fed-batch production bioreactors

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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