43816

DL-Dithiothreitol solution

BioUltra, for molecular biology, ~1 M in H₂O

• Synonym(s): DTT, (±)-threo-1,4-Dimercapto-2,3-butanediol solution
• Empirical Formula (Hill Notation): C₄H₁₀O₂S₂
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• MDL number: MFCD00004877
• PubChem Substance ID: 57650196
• NACRES: NA.25

Properties

• grade: for molecular biology
• product line: BioUltra
• form: liquid
• concentration: ~1 M in H₂O
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH: 2.7-5.0
• density: 1.04 g/mL at 20 °C
• absorption: cut-off at 330 nm
• suitability: in accordance for filter test
• storage temp.: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Description

Application

DL-dithiothreitol solution has been used:

• in the treatment of intestinal explants
• in homogenate preparation of gill cells for enzymatic assays
• in western blotting
• in transglutaminase activity

Biochem/physiol Actions

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H315 - H318
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves