All Photos(1)

Urea

BioUltra, for molecular biology, ≥99% (T)

All Photos(1)

Synonym(s):

Carbamide, Carbonyldiamide

Linear Formula:

NH₂CONH₂

CAS Number:

57-13-6

Molecular Weight:

60.06

Beilstein:

635724

EC Number:

200-315-5

MDL number:

MFCD00008022

PubChem Substance ID:

57651307

NACRES:

NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

assay

≥99% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.1% biuret
<0.005% formaldehyde (HCHO)

ign. residue

≤0.01% (as SO₄)

loss

≤0.1% loss on drying, 20 °C (HV)

pH

8.0-10.0 (20 °C, 8 M in H₂O)

mp

132-135 °C (lit.)

solubility

H₂O: 8 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl^-): ≤5 mg/kg
sulfate (SO₄^2-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

NC(N)=O

λ

8 M in H₂O

UV absorption

λ: 260 nm A_max: ≤0.03
λ: 280 nm A_max: ≤0.02

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759) , CA2(760)
rat ... Ppm1a(24666)

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Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)