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51456

Sigma-Aldrich

Urea

BioUltra, Molecular Biology, 99% (T)

Synonym(s):

Carbamide, Carbonyldiamide

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500 G
€90.40
2.5 KG
€313.00

€90.40


Estimated to ship onJune 23, 2025Details


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500 G
€90.40
2.5 KG
€313.00

About This Item

Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.25
grade:
Molecular Biology

€90.40


Estimated to ship onJune 23, 2025Details


Request a Bulk Order

grade

Molecular Biology

Quality Level

product line

BioUltra

Assay

99% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.1% biuret
<0.005% formaldehyde (HCHO)

ign. residue

≤0.01% (as SO4)

loss

≤0.1% loss on drying, 20 °C (HV)

pH

8.0-10.0 (20 °C, 8 M in H2O)

mp

132-135 °C (lit.)

solubility

H2O: 8 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

NC(N)=O

λ

8 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.03
λ: 280 nm Amax: ≤0.02

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

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1 of 4

This Item
1.04166RDD034U5378
Urea BioUltra, Molecular Biology, 99% (T)

51456

Urea

Urea granulated, EMPROVE&#174; EXPERT, Ph. Eur., BP, ChP, JP, USP, ACS

1.04166

Urea

Urea free-flowing, Redi-Dri&#8482;, Bioreagent, &#8805;98%

RDD034

Urea

Urea powder, BioReagent, Molecular Biology, suitable for cell culture

U5378

Urea

grade

for molecular biology

grade

ACS reagent

grade

-

grade

for molecular biology

Quality Level

200

Quality Level

500

Quality Level

100

Quality Level

200

assay

99% (T)

assay

-

assay

≥98%

assay

≥98%

solubility

H2O: 8 M at 20 °C, clear, colorless

solubility

-

solubility

-

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

form

powder or crystals

form

granular

form

-

form

powder

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent[1]; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides[2][3][4]

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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