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51456

Sigma-Aldrich

Urea

BioUltra, for molecular biology, 99% (T)

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Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

99% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.1% biuret
<0.005% formaldehyde (HCHO)

ign. residue

≤0.01% (as SO4)

loss

≤0.1% loss on drying, 20 °C (HV)

pH

8.0-10.0 (20 °C, 8 M in H2O)

mp

132-135 °C (lit.)

solubility

H2O: 8 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

NC(N)=O

λ

8 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.03
λ: 280 nm Amax: ≤0.02

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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This Item
U5378U6504U0631
Urea BioUltra, for molecular biology, 99% (T)

Sigma-Aldrich

51456

Urea

Premium Grade
Urea powder, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

U5378

Urea

Essential+ Grade
Urea suitable for electrophoresis

Sigma-Aldrich

U6504

Urea

-
Urea BioXtra, pH 7.5-9.5 (20&#160;°C, 5&#160;M in H2O)

Sigma-Aldrich

U0631

Urea

Premium Grade
assay

99% (T)

assay

≥98%

assay

≥98%

assay

98.5-101.5%

form

powder or crystals

form

powder

form

powder or crystals

form

powder, crystals or pellets

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.1% biuret, <0.005% formaldehyde (HCHO)

impurities

-

impurities

≤0.005% Ammonia

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

pH

8.0-10.0 (20 °C, 8 M in H2O)

pH

-

pH

-

pH

7.5-9.5 (20 °C, 5 M in H2O)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7 M urea.
T Maniatis et al.
Methods in enzymology, 65(1), 299-305 (1980-01-01)
R. Poulson et al.
The Ribonucleic Acids, 333-333 (1977)
A Paulus et al.
Electrophoresis, 14(1-2), 27-35 (1993-01-01)
DNA digest analysis with polymer-filled capillaries in capillary electrophoresis is described. The samples analyzed consisted of commercially available standards including a 100 base pair ladder with repeating units up to 2000 base pairs. Three DNA digests covered the most common
R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Florin Sasarman et al.
Human molecular genetics, 24(10), 2841-2847 (2015-02-06)
Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1

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