a 3% peroxide block solution should be used.
925B
Peroxide Block
3% Hydrogen Peroxide
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About This Item
NACRES:
NA.24
UNSPSC Code:
12352200
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description
[For In Vitro Diagnostic Use in Select Regions]
form
aqueous solution
packaging
pkg of 1 L (925B-09), pkg of 100 mL ready to use (925B-04), pkg of 15 mL ready to use (925B-02), pkg of 200 mL ready to use (925B-05), pkg of 50 mL ready to use (925B-03)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry: suitable
shipped in
ambient
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| description [For In Vitro Diagnostic Use in Select Regions] | description - | description - | description - |
| form aqueous solution | form - | form lyophilized powder | form lyophilized |
| packaging pkg of 1 L (925B-09) | packaging kit of 1 (7 components) | packaging - | packaging pkg of 8 mg (~40 mg lyophilizate) |
| manufacturer/tradename Cell Marque® | manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename Roche |
| technique(s) immunohistochemistry: suitable | technique(s) ELISA: suitable, immunoblotting: suitable, immunohistochemistry: suitable | technique(s) direct ELISA: 1:80,000 using human IgG | technique(s) - |
| shipped in ambient | shipped in - | shipped in - | shipped in wet ice |
General description
Peroxide Block is used to block the endogenous peroxide enzymes that naturally occur in tissue sections. This is a necessary step when performing immunohistochemistry and utilizing a horseradish peroxidase enzyme (HRP) label. If this step is eliminated from the protocol, endogenous peroxide enzymes may cause the chromogen to precipitate, thereby causing background staining to occur. Use of this reagent virtually eliminates this possibility.
Physical form
Solution containing hydrogen peroxide
Other Notes
For Technical Service please contact: 800-665-7284 or email: [email protected]
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the % of peroxide in peroxide block 925B-09?
1 answer-
Helpful?
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There is a requirement for large volumes of H2O2 (containers that exceed 1000 mls), please suggest
1 answer-
Peroxide Block is a 3% hydrogen peroxide solution commonly used for performing IHC.
Helpful?
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Can peroxide block as a diluent or how is it used?
1 answer-
The peroxide block is used to neutralize the endogenous peroxidase that arises from using an HRP or Horse Radish Peroxidase kit. It is part of the protocol and is typically used for 10 minutes as one of the steps.
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