Merck
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D0547

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
DME/F-12, 1:1 mixture
MDL number:

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: yes
HEPES: no
L-glutamine: yes
NaHCO3: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

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Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantity

Formulated to contain 12.0 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate

Certificate of Analysis

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Certificate of Origin

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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