Merck
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D1947

Sigma-Aldrich

Diffinity RapidTip®

for PCR Purification

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Synonym(s):
PCR clean up tip, PCR purification tip, PCR reaction clean-up tip
EC Number:
NACRES:
NA.52

Quality Level

form

solid

manufacturer/tradename

(Diffinity Genomics, Inc.)

capacity

50(µL)

storage temp.

room temp

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This Item
HPPCRPKROPCR9604EXPEXTKB
Diffinity RapidTip® for PCR Purification

Sigma-Aldrich

D1947

Diffinity RapidTip®

KAPA Express Extract

Roche

EXPEXTKB

KAPA Express Extract

form

solid

form

-

form

-

form

-

capacity

50(µL)

capacity

-

capacity

-

capacity

-

storage temp.

room temp

storage temp.

-

storage temp.

15-25°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

General description

Diffinity RapidTip effectively removes dNTPs, primers and primer dimers while providing greater than 90% recovery of pure DNA fragments from 100 bp to 10 kb. The functional pipette tip contains everything you need for PCR purification. The tip is filled with a proprietary adsorption technology that has a differential affinity for PCR components. The dispensed solution yields purified, high quality DNA ready for downstream applications such as DNA sequencing, SNP analysis and microarray printing.

Application

Diffinity RapidTip® has been used in PCR product purification.

Features and Benefits

  • Single step
  • Recovers 90% of high quality dsDNA
  • Optimized for 25 μL RXN

Legal Information

RapidTip is a registered trademark of Diffinity Genomics, Inc.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

STOT RE 2 Inhalation

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sarah L Dean et al.
Molecular ecology, 23(6), 1364-1378 (2013-10-12)
Nitrogen (N) deposition rates are increasing globally due to anthropogenic activities. Plant community responses to N are often attributed to altered competitive interactions between plants, but may also be a result of microbial responses to N, particularly root-associated fungi (RAF)
Teruaki Nakatsuji et al.
Nature communications, 4, 1431-1431 (2013-02-07)
Commensal microbes on the skin surface influence the behaviour of cells below the epidermis. We hypothesized that bacteria or their products exist below the surface epithelium and thus permit physical interaction between microbes and dermal cells. Here to test this
Michael J Goblirsch et al.
PloS one, 8(7), e69831-e69831 (2013-07-31)
A major hindrance to the study of honey bee pathogens or the effects of pesticides and nutritional deficiencies is the lack of controlled in vitro culture systems comprised of honey bee cells. Such systems are important to determine the impact
Jason W Sahl et al.
Applied and environmental microbiology, 78(14), 4884-4892 (2012-05-15)
The sequencing and analysis of multiple housekeeping genes has been routinely used to phylogenetically compare closely related bacterial isolates. Recent studies using whole-genome alignment (WGA) and phylogenetics from >100 Escherichia coli genomes has demonstrated that tree topologies from WGA and
Jessamina E Blum et al.
mBio, 4(6), e00860-e00813 (2013-11-07)
We report that establishment and maintenance of the Drosophila melanogaster microbiome depend on ingestion of bacteria. Frequent transfer of flies to sterile food prevented establishment of the microbiome in newly emerged flies and reduced the predominant members, Acetobacter and Lactobacillus

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