Merck
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D2906

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym(s):
DME/F-12, 1:1 mixture, DMEM Hams F12
MDL number:
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
NaHCO3: no
L-glutamine: yes
HEPES: 15 mM

shipped in

ambient

storage temp.

2-8°C

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General description

Suitable for growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Quantity

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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