D8418

Dimethyl sulfoxide

for molecular biology

• Synonym(s): Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
• Linear Formula: (CH₃)₂SO
• CAS Number: 67-68-5
• Molecular Weight: 78.13
• Beilstein: 506008
• EC Number: 200-664-3
• MDL number: MFCD00002089
• PubChem Substance ID: 24894214
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 200
• vapor density: 2.7 (vs air)
• vapor pressure: 0.42 mmHg ( 20 °C)
• product line: BioReagent
• Assay: ≥99.9%
• form: liquid
• autoignition temp.: 573 °F
• shelf life: Recommended retest period - 2 years
• expl. lim.: 42 %, 63 °F
• technique(s): DNA sequencing: suitable; PCR: suitable; transfection: suitable
• impurities: ≤0.001 meq/g Titratable acid; ≤0.1% water (Karl Fischer)
• color: colorless
• refractive index: n20/D 1.479 (lit.)
• bp: 189 °C (lit.)
• mp: 16-19 °C (lit.)
• solubility: H₂O: miscible (completely)
• density: 1.10 g/mL (lit.)
• absorption: passes test
• suitability: suitable for molecular biology
• foreign activity: DNase and RNase, none detected
• storage temp.: room temp
• SMILES string: CS(C)=O
• InChI: 1S/C2H6OS/c1-4(2)3/h1-2H3
• InChI key: IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Description

General description

Features and Benefits:

• Suitable for Molecular Applications
• Nuclease-Free DMSO
• DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
• Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

• polymerase chain reaction (PCR)
• amplification of cDNA libraries
• DNA sequencing
• column-loading buffers for poly(A)+ RNA selection
• buffers for the transformation of competent E. coli
• transfection protocols.

Dimethyl sulfoxide has been used-

• as an oligonucleotide solvent in array spotting[1]
• as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
• for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
• for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
• in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.

To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves