D8437

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): DMEM Hams F12, DME/F-12, 1:1 mixture
• MDL number: MFCD00217417
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: glucose: 3.15 g/L; HEPES: yes; L-glutamine: yes; phenol red: yes; sodium pyruvate: 0.055 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s medium (DMEM), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:

• has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells
• has been used to culture the cartilage biopsies
• may be used for the propagation of Cowdria ruminantium

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable