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EC600

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GenElute-E Single Spin DNA Cleanup Kit

greener alternative
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Synonym(s):
E Single Spin DNA Cleanup Kit, GenElute DNA Cleanup Kit

purified by

(Single-spin negative chromotography)
(Time: 3 minutes or less)

feature

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

DNA purification: suitable

greener alternative category

storage temp.

room temp

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This Item
EC700EC800EC500
storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

purified by

(Single-spin negative chromotography)

purified by

(Single-spin negative chromotography), (Time: 3 minutes or less)

purified by

(Single-spin negative chromotography), (Time: 3 minutes or less)

purified by

(Single-spin negative chromotography), (Time: 45 minutes or less)

feature

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)

feature

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the removal of organic solvent traces from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.)

feature

Compatible Application (Suitable for most common downstream applications, including RT-PCR, gene expression, and NGS), Intended use (For depletion of impurities from RNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.)

feature

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For purification of genomic DNA from plant tissue), Typical/expected yield (Varies by sample. Please reference user guide for more information.)

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned,

greener alternative category

, Aligned

greener alternative category

Aligned,

greener alternative category

, Aligned

General description

The GenElute-E Single Spin DNA Cleanup Kit is a one-step cleanup kit for the depletion of impurities and partial fractions (<50 bp) from pre-purified samples of DNA. The kit efficiently removes salt, detergent, nucleotides, primers, proteins/enzymes, organic solvents and many other inhibitors of downstream applications. The standard protocol, which can be completed in 3 minutes or less, is suitable for DNA fragments, plasmid DNA, and genomic DNA.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

Application

Traditional methods for purifying genomic DNA rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute-E Single Spin DNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, and dyes from DNA solutions. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.

Features and Benefits

Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

Storage and Stability

Use within 12 months of receipt.

Other Notes

We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised.  GenElute-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Articles

Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes

Impact of Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes. Evaluation of the purity of genomic DNA by UV spectrophotometry, gel electrophoresis, and downstream qPCR using GenElute™-E DNA purification kits.

Related Content

GenElute™-E Kits for RNA and DNA Purification

GenElute™-E Single Spin DNA and RNA purification technology enables rapid DNA and RNA extraction and purification from mammalian cells, blood, tissue, and plant tissue samples.

GenElute™-E Single Spin DNA and RNA Purification Kits for Green Labs

Reduce plastic waste and eliminate hazardous liquid waste for more sustainable laboratories with GenElute™-E Single Spin DNA and RNA prep kits.

How to Prep DNA and RNA using GenElute™-E Single Spin Purification Kits

Demo video showing how to purify DNA and RNA GenElute™-E single spin nucleic acid purification kits

GenElute™-E Single Spin DNA and RNA Purification Kits FAQs

Answers to frequently asked questions related to GenElute™-E single spin DNA and RNA purification and negative chromatography

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