Merck
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F6636

SAFC

Nutrient Mixture F-12 Ham

powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture

Synonym(s):
Ham’s F-12
MDL number:
NACRES:
NA.75

form

powder

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

components

phenol red: 0.00125 g/L
glucose: 1.802 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Quantity

Formulated to contain 11.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.68 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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